Coo Ayotunde E, Oviasogie Faith E, Ikhajiagbe Beckley
Department of Microbiology, University of Benin, Nigeria.
Department of Plant Biology and Biotechnology, University of Benin, Nigeria.
BioTechnologia (Pozn). 2022 Dec 24;103(4):385-396. doi: 10.5114/bta.2022.120707. eCollection 2022.
In recent years, an increase in environmental pollution has been observed due to rapid industrialization, unsafe agricultural practices, and increased human activities on energy reservoirs. The wide use of petroleum hydrocarbon products as energy sources has contaminated the soil and the environment, thereby posing serious threats to all life forms, including humans. This study aimed to investigate the role of poultry droppings and pig dung in enhancing the bioremediation of diesel-contaminated soil. Soil samples were collected, processed by air drying and sieving, weighed in experimental bowls (5000 g), and contaminated with 250 ml of diesel. Then, poultry droppings and pig dung were added to the soil samples in different ratios, namely 1 : 1, 1 : 2, and 2 : 1. The diesel-contaminated soil sample without treatment served as the control. Thirty days after exposure to the experimental treatment regimes, the total bacterial count and the hydrocarbon-utilizing bacterial count of the diesel-contaminated soil ranged from 0.4 × 10 to 2.7×10 CFU/g and from 0.1×10 to 2.1×10 CFU/g, respectively. The total fungal count and the hydrocarbon-utilizing fungi count ranged from 0.6 × 10 to 2.1×10 SFU/g and from 0.2×10 to 1.7×10 SFU/g, respectively. sp., sp., and sp were found to be active degraders. A significant reduction in the total aliphatic hydrocarbon (TAH) content of the diesel-contaminated soil was reported, with remediation approaching 95% in 30 days when the poultry droppings - pig dung mixture was added to the soil. The remediation of diesel-contaminated soils is important for the enhancement of the ecosystem. This study has shown that the use of farm waste such as the poultry droppings - pig dung mixture can enhance the remediation of diesel-contaminated soils.
近年来，由于快速工业化、不安全的农业生产方式以及人类对能源储备活动的增加，环境污染有所加剧。作为能源来源的石油烃产品的广泛使用污染了土壤和环境，从而对包括人类在内的所有生命形式构成严重威胁。本研究旨在调查家禽粪便和猪粪在增强柴油污染土壤生物修复中的作用。采集土壤样本，通过风干和筛分进行处理，称取5000克放入实验碗中，并用250毫升柴油进行污染。然后，将家禽粪便和猪粪以不同比例添加到土壤样本中，即1:1、1:2和2:1。未经处理的柴油污染土壤样本作为对照。在接受实验处理方案30天后，柴油污染土壤的总细菌计数和利用烃类的细菌计数分别为0.4×10至2.7×10 CFU/克和0.1×10至2.1×10 CFU/克。总真菌计数和利用烃类的真菌计数分别为0.6×10至2.1×10 SFU/克和0.2×10至1.7×10 SFU/克。发现 sp.、 sp.和 sp.是活跃的降解菌。据报道，柴油污染土壤的总脂肪烃（TAH）含量显著降低，当将家禽粪便 - 猪粪混合物添加到土壤中时，30天内修复率接近95%。柴油污染土壤的修复对于增强生态系统很重要。本研究表明，使用家禽粪便 - 猪粪混合物等农业废弃物可以增强柴油污染土壤的修复。
