Poretsky Leonid, Yeshua Arielle, Cantor Tal, Avtanski Dimiter, Stojchevski Radoslav, Ziskovich Karina, Singer Tomer
Friedman Diabetes Institute, Lenox Hill Hospital, Northwell Health, New York, NY, USA.
Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA.
Metabol Open. 2023 Jan 13;17:100230. doi: 10.1016/j.metop.2023.100230. eCollection 2023 Mar.
Reproduction and energy metabolism are closely related, and fertility can be directly affected by either obesity or malnutrition. In this study, we investigated the effects of irisin and leptin, two hormones primarily involved in energy metabolism, on the expression of genes encoding key steroidogenic enzymes in primary cultures of human granulosa cells. Granulosa cells were purified from follicular fluid samples obtained during fertilization (IVF) procedure, cultured, and treated with irisin (125-2000 ng/ml) or leptin (25-400 ng/ml) for 1-3 days. mRNA expression levels of cytochrome P450 enzymes [CYP11A1, CYP19A1, CYP21A2], hydroxy-delta-5-steroid dehydrogenase, 3 beta and steroid delta-isomerase 1 (HSD3B1), and hydroxysteroid 17-beta dehydrogenase 3 (HSD17B3) were measured using qRT-PCR analysis. Irisin significantly upregulated mRNA levels, while leptin upregulated and mRNA levels. These preliminary results show that irisin and leptin may directly affect the expression of the genes important for ovarian steroidogenesis and female reproduction.
生殖与能量代谢密切相关，肥胖或营养不良均可直接影响生育能力。在本研究中，我们调查了鸢尾素和瘦素这两种主要参与能量代谢的激素，对人颗粒细胞原代培养物中关键类固醇生成酶编码基因表达的影响。颗粒细胞从体外受精（IVF）过程中获得的卵泡液样本中纯化出来，进行培养，并用鸢尾素（125 - 2000 ng/ml）或瘦素（25 - 400 ng/ml）处理1 - 3天。使用qRT-PCR分析测量细胞色素P450酶[CYP11A1、CYP19A1、CYP21A2]、羟基-δ-5-类固醇脱氢酶、3β和类固醇δ-异构酶1（HSD3B1）以及羟基类固醇17-β脱氢酶3（HSD17B3）的mRNA表达水平。鸢尾素显著上调mRNA水平，而瘦素上调和mRNA水平。这些初步结果表明，鸢尾素和瘦素可能直接影响对卵巢类固醇生成和女性生殖重要的基因的表达。
