斯皮克斯盔嘴雉胚胎有产生类固醇激素的潜力。
Galea spixii embryos have potential to produce steroid hormones.
作者信息
de Oliveira Franceliusa Delys, Santos Paulo Ramos da Silva, de Oliveira Moacir Franco, de Assis Antônio Chaves
机构信息
Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.
Departamento de Ciência Animal, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brasil.
出版信息
Anim Reprod. 2023 Jan 16;19(4):e20220091. doi: 10.1590/1984-3143-AR2022-0091. eCollection 2022.
Estrogens and progestogens are hormones produced by maternal organs and it is required for recognition and maintenance of pregnancy. In addition, the embryo may also be a source. For this, the aim was to identify steroidogenic expression on Galea spixii embryos early in the embryonic period. Embryos were collected on Days 10 and 15 of gestation; some were fixed in 4% paraformaldehyde for morphological and immunohistochemical analysis (P450arom), whereas others had RNA extracted to determine presence of CYP19a1 gene. In addition, for immunochemistry, maternal ovaries were collected as positive control tissues. Maternal tissues had positive staining for aromatase, whereas none of the embryos stained for P450 aromatase. Based on qPCR reactions, CYP19a1 gene were expressed in all embryos. Galea spixii embryos expressed steroidogenic genes during the post-implantation period, indicating they have the potential to produce steroid hormones.
雌激素和孕激素是母体器官产生的激素，对于识别和维持妊娠是必需的。此外，胚胎也可能是一个来源。为此，目的是在胚胎期早期鉴定斯氏原狐猴胚胎上的类固醇生成表达。在妊娠第10天和第15天收集胚胎；一些胚胎用4%多聚甲醛固定用于形态学和免疫组织化学分析（P450芳香化酶），而其他胚胎提取RNA以确定CYP19a1基因的存在。此外，对于免疫化学，收集母体卵巢作为阳性对照组织。母体组织对芳香化酶有阳性染色，而没有一个胚胎对P450芳香化酶染色。基于qPCR反应，CYP19a1基因在所有胚胎中均有表达。斯氏原狐猴胚胎在着床后期表达类固醇生成基因，表明它们有产生类固醇激素的潜力。
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