Meshkat Mostafa, Shemshadi Bahar, Amini Kumarss
Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran.
Vet Res Forum. 2022;13(4):597-601. doi: 10.30466/vrf.2021.523112.3128. Epub 2022 Dec 15.
The present study was conducted to investigate the detection and identification of species via molecular techniques and evaluate the serum concentrations of inflammatory factors in species. The fecal samples (n = 256) were collected from pre-weaned (≤ 2.00 months) calves and the positive samples were identified utilizing Ziehl-Neelsen staining. Nested species-specific multiplex PCR (nssm-PCR) and restriction fragment length polymorphism (RFLP) were used to identify the species and sub-species. The serum concentrations of IL-1β, IL-6, IL-12, TNF-α, and IFN-γ were also assessed. The results revealed that 10.54% of samples were positive. The results of Nested-PCR showed that 92.59% of the samples were positive for while 7.41% were positive for . The results of RFLP confirmed 92.59% of the samples for , 3.70% for / , and 3.70% for . The serum concentrations of IL-1β, IL-6, IL-12, TNF-α, and IFN-γ were significantly higher in the infected calves compared to those in healthy calves. However, the serum concentration of IFN-γ was significantly higher in the calves infected with while the serum concentrations of TNF-α and IL-6 were significantly higher in those infected with In conclusion, was prevalent in the region and the calves demonstrated inflammatory responses to species.
本研究旨在通过分子技术调查[物种名称]的检测与鉴定，并评估[物种名称]中炎症因子的血清浓度。从断奶前（≤2.00个月）的犊牛收集粪便样本（n = 256），并利用齐-尼氏染色法鉴定阳性样本。采用巢式物种特异性多重PCR（nssm-PCR）和限制性片段长度多态性（RFLP）来鉴定物种和亚种。还评估了IL-1β、IL-6、IL-12、TNF-α和IFN-γ的血清浓度。结果显示，10.54%的样本呈阳性。巢式PCR结果表明，92.59%的样本对[某种情况]呈阳性，而7.41%对[另一种情况]呈阳性。RFLP结果证实，92.59%的样本为[某种情况]，3.70%为[某种情况]/[另一种情况]，3.70%为[又一种情况]。与健康犊牛相比，感染犊牛的IL-1β、IL-6、IL-12、TNF-α和IFN-γ血清浓度显著更高。然而，感染[某种物种]的犊牛中IFN-γ血清浓度显著更高，而感染[另一种物种]的犊牛中TNF-α和IL-6血清浓度显著更高。总之，[物种名称]在该地区流行，犊牛对[物种名称]表现出炎症反应。
