The profile of inflammatory factors in dairy calves with infection.

The present study was conducted to investigate the detection and identification of species via molecular techniques and evaluate the serum concentrations of inflammatory factors in species. The fecal samples (n = 256) were collected from pre-weaned (≤ 2.00 months) calves and the positive samples were identified utilizing Ziehl-Neelsen staining. Nested species-specific multiplex PCR (nssm-PCR) and restriction fragment length polymorphism (RFLP) were used to identify the species and sub-species. The serum concentrations of IL-1β, IL-6, IL-12, TNF-α, and IFN-γ were also assessed. The results revealed that 10.54% of samples were positive. The results of Nested-PCR showed that 92.59% of the samples were positive for while 7.41% were positive for . The results of RFLP confirmed 92.59% of the samples for , 3.70% for / , and 3.70% for . The serum concentrations of IL-1β, IL-6, IL-12, TNF-α, and IFN-γ were significantly higher in the infected calves compared to those in healthy calves. However, the serum concentration of IFN-γ was significantly higher in the calves infected with while the serum concentrations of TNF-α and IL-6 were significantly higher in those infected with In conclusion, was prevalent in the region and the calves demonstrated inflammatory responses to species.