Ghorbani Alireza, Khoshbakht Rahem, Kaboosi Hami, Shirzad-Aski Hesamaddin, Peyravii Ghadikolaii Fatemeh
Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
Vet Res Forum. 2022;13(4):569-576. doi: 10.30466/vrf.2021.527563.3160. Epub 2022 Dec 15.
Avian pathogenic (APEC) and uropathogenic (UPEC) can cause vast infections in humans and poultry. The present study was conducted to compare the isolates of the APEC and UPEC pathotypes on the basis phenotypic and genotypic features of antibiotic resistance and phylogenetic differences. Total number of 70 identified strains, including 35 APEC and 35 UPEC isolates, were isolated from avian colibacillosis and human urinary tract infection (UTI), and were subjected to the antimicrobial susceptibility testing, polymerase chain reaction (PCR) detection of the resistance genes, phylogenetic grouping and DNA fingerprinting with enterobacterial repetitive intergenic consensus PCR (ERIC - PCR) to survey the variability of the isolates. The most resistance rates among all isolates were, respectively, obtained for Ampicillin (84.20%) and sulfamethoxazole-trimethoprim (65.70%). The APEC and UPEC isolates showed the most susceptibility to imipenem and gentamycin, respectively. Among 70 APEC and UPEC isolates 34.20%, 32.80%, 20.00%, and 12.80% belonged to the A, B2, D, and B1 phylogenetic groups, respectively. Analysis of the DNA fingerprinting phylogenetic tree showed 10 specific clusters of APEC and UPEC isolates. According to the results, the most effective antibiotics and the phenotypic and genotypic predominant resistance patterns of the APEC and UPEC isolates were different. Moreover, APECs and UPECs showed various dominant phylogenetic groups. With all descriptions, the APEC isolates still are potential candidates for carrying important resistance genes and can be one of the possible strains related to human infections.
禽致病性大肠杆菌（APEC）和尿路致病性大肠杆菌（UPEC）可在人类和家禽中引发大量感染。本研究旨在基于抗生素耐药性的表型和基因型特征以及系统发育差异，比较APEC和UPEC致病型的分离株。从禽大肠杆菌病和人类尿路感染（UTI）中分离出总共70株已鉴定菌株，包括35株APEC和35株UPEC分离株，并对其进行抗菌药敏试验、耐药基因的聚合酶链反应（PCR）检测、系统发育分组以及用肠杆菌重复基因间共识PCR（ERIC-PCR）进行DNA指纹图谱分析，以调查分离株的变异性。所有分离株中，氨苄西林（84.20%）和磺胺甲恶唑-甲氧苄啶（65.70%）的耐药率最高。APEC和UPEC分离株分别对亚胺培南和庆大霉素最敏感。在70株APEC和UPEC分离株中，分别有34.20%、32.80%、20.00%和12.80%属于A、B2、D和B1系统发育组。对DNA指纹图谱系统发育树的分析显示，APEC和UPEC分离株有10个特定的聚类。根据结果，APEC和UPEC分离株最有效的抗生素以及表型和基因型的主要耐药模式不同。此外，APEC和UPEC显示出不同的优势系统发育组。综上所述，APEC分离株仍是携带重要耐药基因的潜在候选者，可能是与人类感染相关的菌株之一。
