根据临床和实验室标准协会指南，印度北部明显健康献血者血小板计数及其他血小板指标的参考区间：是否需要重新定义重复血小板单采献血的血小板计数临界值？

Reference interval of platelet counts and other platelet indices in apparently healthy blood donors in North India according to Clinical and Laboratory Standards Institute guidelines: Need to redefine the platelet count cutoffs for repeat plateletpheresis donation?

作者信息

Pabbi Swati, Tiwari Aseem Kumar, Aggarwal Geet, Sharma Govind, Marik Arghyadeep, Luthra Aanchal Sunil, Upadhyay Anand Prakash, Singh Manish Kumar

机构信息

Department of Transfusion Medicine, Medanta-The Medicity, Gurgaon, Haryana, India.

Department of Medanta Institute of Education and Research Medanta-The Medicity, Gurgaon, Haryana, India.

出版信息

Asian J Transfus Sci. 2022 Jul-Dec;16(2):245-250. doi: 10.4103/ajts.AJTS_121_20. Epub 2022 Nov 12.

DOI:10.4103/ajts.AJTS_121_20

PMID:36687539

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9855203/

Abstract

BACKGROUND

In clinical practice, laboratory results are of great importance for the diagnosis and treatment. Reference intervals of different parameters aid health-care professionals in the interpretation of results. There are very few studies on reference intervals from India. This prospective study was conducted to determine the reference intervals for platelet count (PLT) and PLT indices; mean PLT volume (MPV), PLT distribution width (PDW), and PLT large cell ratio (P-LCR). These values can be obtained as a part of a routine complete blood count (CBC) and have diagnostic and prognostic significance in certain diseases. PLT count is an important criterion for the selection of donors for repeat plateletpheresis donation.

MATERIALS AND METHODS

Sixteen hundred and thirty-four first-time healthy volunteer plateletpheresis donors were enrolled for the study. CBC was done, values of PLT, MPV, PDW, and P-LCR were noted, and the results were analyzed. The 95% of the reference distribution was estimated using the 2.5th and 97.5th percentiles following Clinical and Laboratory Standards Institute guidelines. Adverse donor reactions, if any and quality parameters of single donor PLTs (SDP) were also studied.

RESULTS

Reference range values of PLT, MPV, PDW, and P-LCR were 137,825-355,175/μl, 8.1-13.9/fl, 9.1-22.5/fl, and 11.7%-52.9%, respectively, and compared well with other published studies from India. It was observed that reference values of PLT count obtained in the study were lower than reference values that are currently used in most laboratories (150,000-450,000/μl) in India.

CONCLUSION

Based on our results, we are of the opinion that the PLT count cutoffs for repeat plateletpheresis donation may need to be revised downwards for our country which would also mitigate the scarcity of apheresis donors.

摘要

背景

在临床实践中，实验室检查结果对诊断和治疗至关重要。不同参数的参考区间有助于医护人员解读检查结果。印度关于参考区间的研究非常少。本前瞻性研究旨在确定血小板计数（PLT）及血小板指数（平均血小板体积（MPV）、血小板分布宽度（PDW）和血小板大细胞比率（P-LCR））的参考区间。这些值可作为常规全血细胞计数（CBC）的一部分获得，并且在某些疾病中具有诊断和预后意义。血小板计数是选择重复单采血小板献血者的重要标准。

材料与方法

1634名首次参加单采血小板的健康志愿者被纳入本研究。进行全血细胞计数，记录血小板、平均血小板体积、血小板分布宽度和血小板大细胞比率的值，并对结果进行分析。按照临床和实验室标准协会的指南，使用第2.5百分位数和第97.5百分位数估计参考分布的95%。还研究了献血者的不良反应（如有）以及单采血小板（SDP）的质量参数。

结果

血小板、平均血小板体积（MPV）、血小板分布宽度（PDW）和血小板大细胞比率（P-LCR）的参考范围值分别为137,825 - 355,175/μl、8.1 - 13.9/fl、9.1 - 22.5/fl和11.7% - 52.9%，与印度其他已发表的研究结果比较良好。观察到本研究中获得的血小板计数参考值低于印度目前大多数实验室使用的参考值（15,000 - 450,000/μl）。