Sachdev Ritesh, Tiwari Aseem K, Goel Shalini, Raina Vimarsh, Sethi Monisha
Department of Pathology, Lab Medicine and Transfusion Medicine, Medanta-The Medicity Hospital, Gurgaon, Haryana, India.
Indian J Pathol Microbiol. 2014 Apr-Jun;57(2):231-5. doi: 10.4103/0377-4929.134676.
This study aims to establish biological reference interval for novel platelet parameters.
A total of 945 healthy individuals, age ranges from 18 to 64 years (881 males and 64 females) coming for voluntary blood donation from June to August 2012 (3 months) were enrolled after exclusion of rejection criteria.
The samples were assayed by running in complete blood count + reticulocyte mode on the Sysmex XE-2100 hematology analyzer and the reference interval for the population was calculated using Clinical and Laboratory Standards Institute guidelines.
Tests were performed using SPSS (Statistical Product and Service Solutions, developed by IBM corporation), version 13. Student t test and pearsons correlation analysis were also used.
The normal range for various parameters was platelet count: 150-520 × 10(3)/cu mm, immature platelet fraction (IPF): 0.3-8.7%, platelet distribution width (PDW): 8.3-25.0 fL, mean platelet volume (MPV): 8.6-15.5 fL, plateletcrit (PCT): 0.15-0.62%, high immature platelet fraction (H-IPF): 0.1-2.7%, platelet large cell ratio (P-LCR): 11.9-66.9% and platelet-X (PLT-X) (ch): 11.0-22.0. Negative correlation was observed between platelet count (r = -0.468 to r = -0.531; P < 0.001) and PCT (r = -0.080 to r = -0.235; P < 0.05 to P < 0.001) with IPF, PDW, MPV, H-IPF, P-LCR, and platelet-X. IPF/H-IPF showed a positive correlation among them and also with PDW, MPV, P-LCR, platelet-X (r = +0.662 to r = +0.925; P < 0.001).
These novel platelet parameters offer newer avenues in research and clinical use. Establishing biological reference interval for different platelet parameters would help determine true high and low values and help guide treatment decisions.
本研究旨在建立新型血小板参数的生物学参考区间。
2012年6月至8月（3个月）期间前来自愿献血的945名健康个体，年龄在18至64岁之间（男性881名，女性64名），在排除排除标准后被纳入研究。
样本在Sysmex XE - 2100血液分析仪上采用全血细胞计数+网织红细胞模式进行检测，并根据临床和实验室标准协会的指南计算总体的参考区间。
使用SPSS（由IBM公司开发的统计产品与服务解决方案）13版进行测试。还使用了学生t检验和皮尔逊相关分析。
各参数的正常范围为血小板计数：150 - 520×10³/立方毫米，未成熟血小板比率（IPF）：0.3 - 8.7%，血小板分布宽度（PDW）：8.3 - 25.0飞升，平均血小板体积（MPV）：8.6 - 15.5飞升，血小板压积（PCT）：0.15 - 0.62%，高未成熟血小板比率（H - IPF）：0.1 - 2.7%，血小板大细胞比率（P - LCR）：11.9 - 66.9%，血小板 - X（PLT - X）（ch）：11.0 - 22.0。血小板计数（r = -0.468至r = -0.531；P < 0.001）和PCT（r = -0.080至r = -0.235；P < 0.05至P < 0.001）与IPF、PDW、MPV、H - IPF、P - LCR和血小板 - X之间呈负相关。IPF/H - IPF在它们之间以及与PDW、MPV、P - LCR、血小板 - X之间呈正相关（r = +0.662至r = +0.925；P < 0.001）。
这些新型血小板参数为研究和临床应用提供了新途径。建立不同血小板参数的生物学参考区间将有助于确定真正的高值和低值，并有助于指导治疗决策。
