Verma K K, Sanghi S K, Jain A, Gupta D
Department of Chemistry, Rani Durgavati University, Jabalpur, India.
J Pharm Sci. 1987 Jul;76(7):551-3. doi: 10.1002/jps.2600760713.
The accuracy of the measurement of aspirin by high-performance liquid chromatography (HPLC) is reduced by its hydrolysis into salicylic and acetic acids during sample preparation. The ready and quantitative transacetylation of 3-aminophenol by aspirin, giving 3-hydroxyacetanilide (which is stable), has been utilized as a pre-column reaction for the determination of aspirin either alone or in the presence of acetaminophen and caffeine by reversed-phase HPLC.
在样品制备过程中，阿司匹林会水解成水杨酸和乙酸，这会降低高效液相色谱法（HPLC）测定阿司匹林的准确性。阿司匹林能使3-氨基酚迅速定量地发生转乙酰化反应，生成3-羟基乙酰苯胺（该物质稳定），此反应已被用作柱前反应，通过反相高效液相色谱法单独测定阿司匹林，或在对乙酰氨基酚和咖啡因存在的情况下测定阿司匹林。
