聚吡咯纳米颗粒对腐霉的杀真菌活性。

Oomicidal activity of polypyrrole nanoparticles against Pythium insidiosum.

机构信息

Universidade Federal de Santa Maria (UFSM), Santa Maria, RS 97105-900, Brasil.

Universidade Regional Integrada do Alto Uruguai e das Missões (URI), Santiago, RS 97711-500, Brasil.

出版信息

Lett Appl Microbiol. 2023 Jan 23;76(1). doi: 10.1093/lambio/ovac020.

DOI:10.1093/lambio/ovac020

PMID:36688756

Abstract

This study evaluated in-vitro action of a new molecule, the polypyrrole nanoparticles (Ppy-NP), against Pythium insidiosum isolates using M38-A2/CLSI; the minimal inhibitory (MIC) and minimal oomicidal (MOC) concentrations were also determined. Additionally, changes in the hyphae wall of P. insidiosum CBS 575.85 treated with Ppy-NP were evaluated by scanning electron microscopy (SEM). The MIC100 and MOC for all isolates ranged from 8 to 32 μg mL-1, and the MIC90 and MIC50 were 16 μg mL-1. The SEM showed structural damage to the hyphae of P. insidisoum treated with Ppy-NP, as hyphae surfaces with less turgidity were found, thereby showing scaling and ruptures compared to the control (untreated hyphae). Our findings highlighted the anti-P. insidiosum properties of Ppy-NP proved to be a promising candidate for research using pythiosis experimental models.

摘要

本研究采用 M38-A2/CLSI 评估了新分子聚吡咯纳米颗粒（Ppy-NP）对皮炎芽生菌分离株的体外作用，还测定了最小抑菌（MIC）和最小杀真菌（MOC）浓度。此外，还通过扫描电子显微镜（SEM）评估了 Ppy-NP 处理后的皮炎芽生菌 CBS 575.85 菌丝壁的变化。所有分离株的 MIC100 和 MOC 范围为 8 至 32μg mL-1，MIC90 和 MIC50 为 16μg mL-1。SEM 显示，用 Ppy-NP 处理的皮炎芽生菌的菌丝受到结构损伤，因为发现菌丝表面的膨胀度降低，与对照（未处理的菌丝）相比，出现了褶皱和破裂。我们的研究结果强调了 Ppy-NP 的抗皮炎芽生菌特性，证明它是研究皮炎芽生菌实验模型的有前途的候选药物。