体外检测内脐蠕孢真菌游动孢子和菌丝对各种抗真菌药物的敏感性。
In vitro susceptibility of zoospores and hyphae of Pythium insidiosum to antifungals.
机构信息
Programa de Pós-graduação em Veterinária, Universidade Federal de Pelotas, Pelotas, Brazil Laboratório de Micologia, Departamento de Doenças Infecciosas, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, Brazil.
Programa de Pós-graduação em Parasitologia, Universidade Federal de Pelotas, Pelotas, Brazil Laboratório de Micologia, Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas, Pelotas, Brazil
出版信息
J Antimicrob Chemother. 2014 Jun;69(6):1564-7. doi: 10.1093/jac/dku021. Epub 2014 Feb 11.
OBJECTIVES
The purpose of this study was to compare the in vitro susceptibilities of 22 Brazilian isolates of Pythium insidiosum to antifungals using a standardized inoculum of zoospores and a proposed novel inoculum prepared from cultured mycelia (hyphae) of P. insidiosum.
METHODS
A zoospore suspension of P. insidiosum was obtained by the zoosporogenesis technique. The hyphal inoculum was prepared from a suspension of P. insidiosum mycelium. Susceptibility to each drug was evaluated using the CLSI M38-A2 method.
RESULTS
Of the 88 MIC comparisons performed, 36 (41%) showed the same MIC value for the two inocula. The agreement (differences not greater than one dilution) between MICs obtained with both types of inocula was 39.8% (35/88). In other MIC comparisons analysed, 17 (19.3%) showed differences of two or three dilutions.
CONCLUSIONS
We conclude that the use of hyphal inocula of P. insidiosum for in vitro susceptibility tests could be a suitable method for evaluating antimicrobial susceptibility, particularly when it is not possible to obtain a standardized zoospore inoculum.
目的
本研究旨在比较 22 株巴西产皮炎霉（Pythium insidiosum）分离株对 22 株巴西产皮炎霉（Pythium insidiosum）分离株的体外药敏试验结果，采用标准化游动孢子接种和新提议的从皮炎霉培养菌丝（菌丝）制备的接种体。
方法
采用游动孢子发生技术获得皮炎霉游动孢子悬浮液。菌丝接种体是从皮炎霉菌丝悬浮液中制备的。采用 CLSI M38-A2 方法评估每种药物的敏感性。
结果
在进行的 88 次 MIC 比较中，有 36 次（41%）两种接种体的 MIC 值相同。两种接种体获得的 MIC 值之间的一致性（差异不超过一个稀释度）为 39.8%（35/88）。在分析的其他 MIC 比较中，有 17 次（19.3%）显示出两种或三种稀释度的差异。
结论
我们得出结论，使用皮炎霉的菌丝接种体进行体外药敏试验可能是一种评估抗微生物敏感性的合适方法，特别是在无法获得标准化游动孢子接种体时。
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