Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
Institute of Respiratory Diseases of Sun Yat-Sen University, Guangzhou, China.
Int Arch Allergy Immunol. 2023;184(5):460-470. doi: 10.1159/000528129. Epub 2023 Jan 23.
circRNA played a role in a variety of diseases. This paper aimed to explore the differentially expressed circRNA in induced sputum cells of asthma patients, so as to provide new potential biomarkers and new ideas for the study of asthma.
All subjects were from the First Affiliated Hospital of Sun Yat-sen University. Differentially expressed circRNAs of asthma patients were screened by high-throughput sequencing. qRT-PCR was used to verify the expression of differential circRNAs. The association between circRNA and asthma was explored by analyzing the correlation between circRNA and clinical data and some cytokines of asthma patients. The possible ceRNA network was analyzed and predicted by online software, and the expression of each molecule in the network was preliminary verified by qRT-PCR in induced sputum cells and 16HBE cell.
We screened a total of 49 circRNAs differentially expressed in asthma patients (including 12 circRNAs with elevated expression and 37 circRNAs with decreased expression), among which has_circSORT1 and has_circSERPINB1 were significantly elevated. Correlation analysis showed that has_circSORT1 was correlated with FeNO, EOS%, IL-17A, IFN-γ, and PC20, and has_circSERPINB1 was correlated with IL-6, IL-17A, IFN-γ, FEV1%, and FVC%. The possible existence of has_circSORT1/has-miR-185-3p/ZNFX1, a ceRNA regulatory network, in induced sputum cells of asthma patients was hypothesized by online software prediction and qRT-PCR in sputum cells and 16HBE.
Differentially expressed circRNAs existed in induced sputum cells of asthma patients, among which has_circSORT1 and has_circSERPINB1 were significantly upregulated and may be involved in the process of asthma disease, which could be expected to be a potential biomarker for asthma diagnosis. In addition, a ceRNA regulatory network, has_circSORT1/has-miR-185-3p/ZNFX1, may exist in asthma.
circRNA 在多种疾病中发挥作用。本研究旨在探讨哮喘患者诱导痰细胞中差异表达的 circRNA，为哮喘的研究提供新的潜在生物标志物和新的思路。
所有研究对象均来自中山大学附属第一医院。通过高通量测序筛选哮喘患者差异表达的 circRNA。qRT-PCR 用于验证差异 circRNA 的表达。通过分析 circRNA 与哮喘患者临床资料和某些细胞因子的相关性，探讨 circRNA 与哮喘的关系。通过在线软件分析和预测可能的 ceRNA 网络，并初步通过 qRT-PCR 在诱导痰细胞和 16HBE 细胞中验证网络中每个分子的表达。
我们共筛选出哮喘患者差异表达的 49 个 circRNA（包括上调的 12 个 circRNA 和下调的 37 个 circRNA），其中 has_circSORT1 和 has_circSERPINB1 显著上调。相关性分析表明，has_circSORT1 与 FeNO、EOS%、IL-17A、IFN-γ和 PC20 相关，has_circSERPINB1 与 IL-6、IL-17A、IFN-γ、FEV1%和 FVC%相关。通过在线软件预测和 qRT-PCR 在痰细胞和 16HBE 中验证，假设哮喘患者诱导痰细胞中存在 has_circSORT1/has-miR-185-3p/ZNFX1 的 ceRNA 调控网络。
哮喘患者诱导痰细胞中存在差异表达的 circRNA，其中 has_circSORT1 和 has_circSERPINB1 显著上调，可能参与哮喘疾病的发生过程，有望成为哮喘诊断的潜在生物标志物。此外，哮喘患者可能存在 has_circSORT1/has-miR-185-3p/ZNFX1 的 ceRNA 调控网络。
