Kazemi Mohsen, Jahanbin Kambiz, Ojagh Seyed Mahdi, Abdollahi Mehdi
Gorgan University of Agricultural Sciences and Natural Resources, Faculty of Fisheries and Environmental Science, Department of Seafood Science and Technology, Gorgan, Iran.
Shahrood University of Technology, Faculty of Agricultural Engineering, Department of Food Science and Technology, Shahrood, Iran.
Int J Biol Macromol. 2023 Mar 15;231:123384. doi: 10.1016/j.ijbiomac.2023.123384. Epub 2023 Jan 21.
A new generation of antimicrobial film was developed by incorporation of ulvan extracted from Ulva intestinalis into gelatin from common carp scale and its water sensitivity was reduced with addition of beeswax. Optimum composition of gelatin (0-100%w/w), ulvan (0-100%w/w) and beeswax (0-10%w/w) for achieving composite films with minimum water solubility (S) and water vapor permeability (WVP) and maximum tensile strength (TS), elongation at break point (EAB) and antibacterial effect on E. coli (EC) were investigated using mixture design methodology. Both pure gelatin and ulvan films and their composites had relatively good mechanical and optical properties. Addition of ulvan to gelatin produced composite films with good antibacterial properties but water resistance of all the films was weak. Addition of beeswax up to ∼5 % improved the water resistance and mechanical properties of the films without jeopardizing their antibacterial properties. The final optimum formulation with a desirability of 0.709 was achieved as 52.18 % of gelatin, 40.83 % of ulvan and 6.97 % of beeswax resulting in a minimum possible S (40 %) and WVP (1.86 10 g/ms Pa) and maximum possible TS (6.23 MPa) and EAB (89 %) with good EC (7.66 mm). Finally, good mechanical, thermal and microstructural properties of the optimum composite film was confirmed. Altogether, a combination of ulvan and beeswax can be a promising solution for development of gelatin films with both antimicrobial properties and lower water sensitivity.
通过将从肠浒苔中提取的岩藻聚糖硫酸酯掺入鲤鱼鳞片明胶中，开发了新一代抗菌薄膜，并通过添加蜂蜡降低了其水敏感性。使用混合设计方法研究了明胶（0 - 100%w/w）、岩藻聚糖硫酸酯（0 - 100%w/w）和蜂蜡（0 - 10%w/w）的最佳组成，以制备具有最低水溶性（S）和水蒸气透过率（WVP）以及最高拉伸强度（TS）、断裂伸长率（EAB）和对大肠杆菌（EC）抗菌效果的复合薄膜。纯明胶薄膜、纯岩藻聚糖硫酸酯薄膜及其复合材料均具有相对良好的机械性能和光学性能。向明胶中添加岩藻聚糖硫酸酯制备的复合薄膜具有良好的抗菌性能，但所有薄膜的耐水性都较差。添加高达约5%的蜂蜡可改善薄膜的耐水性和机械性能，而不影响其抗菌性能。最终获得了可取性为0.709的最佳配方，即52.18%的明胶、40.83%的岩藻聚糖硫酸酯和6.97%的蜂蜡，从而实现了尽可能低的S（40%）和WVP（1.86×10⁻⁶g/（m·s·Pa））以及尽可能高的TS（6.23MPa）和EAB（89%），同时对大肠杆菌具有良好的抗菌效果（抑菌圈直径为7.66mm）。最后，证实了最佳复合薄膜具有良好的机械性能、热性能和微观结构性能。总之，岩藻聚糖硫酸酯和蜂蜡的组合可能是开发具有抗菌性能和较低水敏感性的明胶薄膜的一个有前景的解决方案。
