College of Life Science, Jilin Agricultural University, Changchun, Jilin 130118, China; College of Food Science and Engineering, Jilin Agricultural University, Changchun, Jilin 130118, China; National Engineering Research Center for Wheat and Corn Deep Processing, Changchun, Jilin 130118, China.
College of Life Science, Jilin Agricultural University, Changchun, Jilin 130118, China.
Biochim Biophys Acta Mol Cell Biol Lipids. 2023 Apr;1868(4):159287. doi: 10.1016/j.bbalip.2023.159287. Epub 2023 Jan 21.
Non-alcoholic fatty liver disease (NAFLD) is a common liver disorder worldwide and a risk factor for obesity and diabetes. Emerging evidence has shown that ferroptosis is involved in the progression of NAFLD. Zeaxanthin (ZEA) is a carotenoid found in human serum. It has been reported that ZEA can ameliorate obesity, prevent age-related macular degeneration, and protect against non-alcoholic steatohepatitis. However, no study has focused on the protective effects of ZEA against NAFLD. In this study, free fatty acid (FFA) induced HepG2 cells were used as a cell model for NAFLD. Our results suggest that ZEA exerts antioxidative and anti-inflammatory effects in FFA-induced HepG2 cells. Moreover, ZEA acted as a ferroptosis inhibitor, significantly reducing reactive oxygen species (ROS) generation and iron overload and improving mitochondrial dysfunction in FFA-induced HepG2 cells. In addition, ZEA downregulated the expression of p53 and modulated downstream targets, such as GPX4, SLC7A11, SAT1, and ALOX15, which contributed to the reduction in cellular lipid peroxidation. Our findings suggest that ZEA has the potential for NAFLD intervention.
非酒精性脂肪性肝病（NAFLD）是一种全球范围内常见的肝脏疾病，也是肥胖症和糖尿病的一个风险因素。新出现的证据表明，铁死亡与 NAFLD 的进展有关。玉米黄质（ZEA）是一种存在于人体血清中的类胡萝卜素。据报道，ZEA 可以改善肥胖症，预防年龄相关性黄斑变性，并防止非酒精性脂肪性肝炎。然而，尚无研究关注 ZEA 对 NAFLD 的保护作用。在这项研究中，使用游离脂肪酸（FFA）诱导的 HepG2 细胞作为 NAFLD 的细胞模型。我们的结果表明，ZEA 在 FFA 诱导的 HepG2 细胞中发挥抗氧化和抗炎作用。此外，ZEA 作为铁死亡抑制剂，可显著减少活性氧（ROS）的产生和铁过载，并改善 FFA 诱导的 HepG2 细胞中线粒体功能障碍。此外，ZEA 下调了 p53 的表达，并调节下游靶点，如 GPX4、SLC7A11、SAT1 和 ALOX15，这有助于减少细胞脂质过氧化。我们的研究结果表明，ZEA 具有干预 NAFLD 的潜力。
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