i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.
Commun Biol. 2023 Jan 23;6(1):91. doi: 10.1038/s42003-023-04426-9.
Cilia assembly and function require intraflagellar transport (IFT), a mechanism that uses “trains” to transport cargoes into and out of cilia. While much has been learned about IFT in the past decades, IFT train assembly, loading of cargo and transport regulation have remained poorly understood. In a recent study, Hesketh, Mukhopadhyay and colleagues obtained the complete structure of the IFT-A complex, a key element of IFT trains. By modelling IFT-A into anterograde trains and performing structure-guided mutagenesis, the authors uncover how the IFT-A complex polymerizes and forms carriages to accomplish its distinctive functions.
纤毛的组装和功能需要内鞭毛运输（IFT），这是一种使用“列车”将货物进出纤毛的机制。尽管在过去几十年中对 IFT 有了很多了解，但 IFT 列车的组装、货物的装载和运输调节仍然知之甚少。在最近的一项研究中，Hesketh、Mukhopadhyay 和同事获得了 IFT-A 复合物的完整结构，IFT-A 复合物是 IFT 列车的关键组成部分。通过将 IFT-A 模型构建成正向列车并进行结构导向的突变，作者揭示了 IFT-A 复合物如何聚合并形成运输工具来完成其独特的功能。
