Jena Biswajit, Biswal Bhagyashree, Sarangi Ashirbad, Giri Alok Kumar, Bhattacharya Debapriya, Acharya Laxmikanta
Molecular Biology and Genetic Engineering Lab, Center for Biotechnology, Siksha O Anusandhan (Deemed to be University), Bhubaneswar, Odisha, India.
Chem Biodivers. 2023 Mar;20(3):e202200691. doi: 10.1002/cbdv.202200691. Epub 2023 Feb 9.
Plectranthus amboinicus (Lour.) Spreng, known as the Indian borage or Mexican mint, is one of the most documented species in the family Lamiaceae for its therapeutic and pharmaceutical values. It is found in the tropical and subtropical regions of the world. The leaf essential oil has immense medicinal benefits like treating illnesses of the skin and disorders like colds, asthma, constipation, headaches, coughs, and fevers. After analyzing earlier reports with regard to the quantity and quality of leaf oil yield, we discovered that the germplasm taken from Odisha is preferable to other germplasms. The objective of the present work is to evaluate the free radical scavenging activity and bactericidal effect of leaf essential oil (EO) of Plectranthus amboinicus (Lour.) Spreng collected from the state of Odisha, India. The hydro distillation technique has been used for essential oil extraction. Upon GC/MS analysis, approximately 57 compounds were identified with Carvacrol as the major compound (peak area=20.25 %), followed by p-thymol (peak area=20.17 %), o-cymene (peak area=19.41 %) and carene (peak area=15.89 %). On evaluation of free radical scavenging activity, it was recorded that the best value of inhibitory concentration, was for DPPH with IC =18.64 ppm and for H O with IC =9.35 ppm. The EO showed efficient bactericidal effect against both gram positive (Mycobacterium smegmatis, Staphylococcus aureus, Enterococcus faecium) and gram negative (Escherichia coli, Vibrio cholerae, Klebsiella pneumoniae) bacteria studied through well diffusion method. Fumigatory action of the essential oil was found against M. smegmatis, the model organism for tuberculosis study. Alamar Blue assay, gave a result with MIC value for M. smegmatis i. e., 0.12 μg/ml and the MBC value of 0.12 μg/ml. Hence, P. amboinicus found in Odisha can be suggested as an elite variety and should be further investigated for efficient administration in drug formulation.
皱叶留兰香（Plectranthus amboinicus (Lour.) Spreng），又名印度琉璃苣或墨西哥薄荷，因其治疗和药用价值，是唇形科中记录最为详尽的物种之一。它分布于世界热带和亚热带地区。其叶精油具有巨大的药用功效，如治疗皮肤病以及感冒、哮喘、便秘、头痛、咳嗽和发烧等疾病。在分析了先前关于叶油产量的数量和质量的报告后，我们发现取自奥里萨邦的种质优于其他种质。本研究的目的是评估从印度奥里萨邦采集的皱叶留兰香叶精油（EO）的自由基清除活性和杀菌效果。采用水蒸馏技术提取精油。通过气相色谱/质谱分析，鉴定出约57种化合物，其中香芹酚为主要化合物（峰面积 = 20.25%），其次是对百里香酚（峰面积 = 20.17%）、邻伞花烃（峰面积 = 19.41%）和蒈烯（峰面积 = 15.89%）。在评估自由基清除活性时，记录到抑制浓度的最佳值，对DPPH而言IC = 18.64 ppm，对H₂O₂而言IC = 9.35 ppm。通过打孔扩散法研究发现，该精油对革兰氏阳性菌（耻垢分枝杆菌、金黄色葡萄球菌、粪肠球菌）和革兰氏阴性菌（大肠杆菌、霍乱弧菌、肺炎克雷伯菌）均显示出有效的杀菌作用。发现该精油对结核研究的模式生物耻垢分枝杆菌有熏蒸作用。alamar蓝法测定结果显示，耻垢分枝杆菌的MIC值即0.12 μg/ml，MBC值为0.12 μg/ml。因此，奥里萨邦发现的皱叶留兰香可被推荐为优良品种，应进一步研究其在药物制剂中的有效应用。
