Nahidi Yalda, Tayyebi Meibodi Naser, Ghazvini Kiarash, Arabi Moghadam Halime Sadat, Akhlaghi Saeed, Torabian Farnaz
Department of Dermatology, Cutaneous Leishmaniasis Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Pathology, Cutaneous Leishmaniasis Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Parasitol. 2022 Oct-Dec;17(4):573-582. doi: 10.18502/ijpa.v17i4.11285.
Cutaneous leishmaniasis (CL) is a chronic granulomatous dermatitis (CGD). Approximately, 90% of CL patients are from seven countries including Iran. We explain PCR) diagnostic technique for chronic granulomatosis dermatoses including CL in Mashhad, Iran.
This study enrolled 64 patients within 2009-2013 with chronic granulomatosis dermatitis referred to dermatology and pathology departments of Imam Reza Hospital, affiliated to Mashhad University of Medical Sciences (MUMS), Mashhad, Iran. We gathered demographic data from archived folders. Histological light microscopic evaluation and parasitological tests were done on selected specimens. We used PCR diagnostic test on specimens. Statistical analysis was done by SPSS version 15.
Generally, 7 out of 64 specimens had DNA and other samples had no DNA. The mean age of patients was 46 ± 18.77 years; disease duration was 7 ± 6.73 months ranging from 1-24 months. Most of the lesions were located on face and upper limb. Totally, 5 out of 7 samples were and 2 out of 7 samples were . Tuberculoid granuloma was present in samples and 3 of samples. Other light microscopic changes were as follow: 42 suppurative granuloma, and epidermal changes including atrophy, pseudoepitheliomatous hyperplasia, and parakeratosis with dermal changes including, plasma cell, involvement of papillary and reticular dermis, and distribution of granuloma to hypodermis.
Our results addressed PCR-based diagnosis of chronic cutaneous leishmaniasis, which is resulted from and .
皮肤利什曼病（CL）是一种慢性肉芽肿性皮炎（CGD）。大约90%的CL患者来自包括伊朗在内的七个国家。我们阐述了在伊朗马什哈德用于诊断包括CL在内的慢性肉芽肿性皮肤病的聚合酶链反应（PCR）技术。
本研究纳入了2009年至2013年间转诊至伊朗马什哈德医科大学（MUMS）附属伊玛目礼萨医院皮肤科和病理科的64例慢性肉芽肿性皮炎患者。我们从存档文件夹中收集人口统计学数据。对选定标本进行组织学光镜评估和寄生虫学检测。我们对标本进行了PCR诊断检测。使用SPSS 15版进行统计分析。
总体而言，64个标本中有7个含有利什曼原虫DNA，其他样本未检测到利什曼原虫DNA。患者的平均年龄为46±18.77岁；病程为7±6.73个月，范围为1至24个月。大多数病变位于面部和上肢。7个样本中，5个为热带利什曼原虫，2个为婴儿利什曼原虫。7个热带利什曼原虫样本中有5个出现结核样肉芽肿，7个婴儿利什曼原虫样本中有3个出现结核样肉芽肿。其他光镜下改变如下：42个出现化脓性肉芽肿，表皮改变包括萎缩、假上皮瘤样增生和角化不全，真皮改变包括浆细胞浸润、乳头层和网状层真皮受累以及肉芽肿延伸至皮下组织。
我们的结果阐述了基于PCR的慢性皮肤利什曼病诊断方法，该病由热带利什曼原虫和婴儿利什曼原虫引起。
