Nishimura Akira, Tanahashi Ryoya, Oi Tomoki, Kan Kyoyuki, Takagi Hiroshi
Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara, Japan.
Department of Food Science and Technology, University of California Davis, One Shields Ave, Davis, CA, USA.
Biosci Biotechnol Biochem. 2023 Mar 21;87(4):458-462. doi: 10.1093/bbb/zbad008.
The current CRISPR/Cas9 systems in the yeast Saccharomyces cerevisiae cannot be considered a non-genetic modification technology because it requires the introduction of Cas9 and sgRNA into yeast cells using plasmid expression systems. Our present study showed that the yeast genome can be edited without plasmid expression systems by using a commercially available protein transfection reagent and chemically modified sgRNAs.
目前酿酒酵母中的CRISPR/Cas9系统不能被视为一种非基因编辑技术，因为它需要使用质粒表达系统将Cas9和sgRNA导入酵母细胞。我们目前的研究表明，通过使用市售的蛋白质转染试剂和化学修饰的sgRNA，可以在不使用质粒表达系统的情况下对酵母基因组进行编辑。
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