Department of Microbiology, Faculty of Life Sciences, Bayero University Kano, PMB 3011, Kano Nigeria.
Project group P2, Robert Koch Institute, Burgstr. 37, D-38855 Wernigerode, Germany.
Lett Appl Microbiol. 2023 Feb 16;76(2). doi: 10.1093/lambio/ovac051.
The opportunistic pathogen Acinetobacter baumannii occupies niches in human and veterinary clinics and other environments. This comparative study was designed to assess the recovery rate of Ac. baumannii from 20 soil samples collected in Germany (from which Ac. baumannii had been previously isolated) on selective CHROMagar Acinetobacter (CMA) and MacConkey Agar (MCA). Suspected Ac. baumannii were confirmed by detection and sequencing of blaOXA-51-like genes. Overall, the recovery rate of Ac. baumannii from both media was similar. Out of the 20 soil samples enriched in mineral salt medium (MM) plus 0.2% acetate for 5 h, Ac. baumannii was recovered from 12 (60%) on CMA and 11 (55%) on MCA, and from 14 (70%) with both selective media after enrichment for 24 h. Typical and distinct colonies of Ac. baumannii were more often observed on CMA with soil enriched in MM for 5 h, while on MCA distinct colonies were more obvious after 24 h. In five soil samples (25%), strains harbouring different OXA-51-like variants were recovered on MCA. Late lactose fermentation (48 h) was observed on MCA. The study suggests that no single growth medium would efficiently recover Ac. baumannii from all soil samples.
机会性病原体鲍曼不动杆菌占据了人类和兽医诊所及其他环境中的小生境。本比较研究旨在评估从德国采集的 20 个土壤样本（先前已从这些土壤样本中分离出鲍曼不动杆菌）在选择性 CHROMagar 不动杆菌（CMA）和麦康凯琼脂（MCA）上回收鲍曼不动杆菌的回收率。通过检测和测序 blaOXA-51 样基因来确认可疑的鲍曼不动杆菌。总的来说，两种培养基回收鲍曼不动杆菌的回收率相似。在 5 小时富集于无机盐培养基（MM）加 0.2%醋酸盐的 20 个土壤样本中，CMA 上回收了 12 个（60%），MCA 上回收了 11 个（55%），24 小时后两种选择性培养基均回收了 14 个（70%）。在 5 小时 MM 富集的土壤中，CMA 上更常观察到典型和独特的鲍曼不动杆菌菌落，而在 MCA 上 24 小时后则更明显。在 5 个土壤样本（25%）中，在 MCA 上回收了携带不同 blaOXA-51 样变体的菌株。在 MCA 上观察到迟发性乳糖发酵（48 小时）。该研究表明，没有单一的生长培养基可以有效地从所有土壤样本中回收鲍曼不动杆菌。
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