Department of Epidemiology and Public Health, University of Maryland School of Medicine, 685 W. Baltimore St, Baltimore, MD 21201, USA.
Eur J Clin Microbiol Infect Dis. 2011 Nov;30(11):1425-30. doi: 10.1007/s10096-011-1237-7. Epub 2011 Apr 12.
The objective of this study was to evaluate the performance of CHROMagar Acinetobacter when compared to sheep blood agar, MacConkey agar and MacConkey agar with 6 μg/ml of imipenem for the detection of A. baumannii in surveillance cultures of hospitalized patients. We utilized peri-anal swabs and sputum samples from patients admitted to the University of Maryland Medical Center ICUs from December 7 through December 21, 2009. Samples were plated onto four media in the following order: (1) 5% sheep blood agar (SBA), (2) MacConkey agar, (3) MacConkey agar with 6 μg/ml of imipenem, and (4) CHROMagar Acinetobacter (CHROMagar). SBA was the gold standard to which all media was compared. There were 165 samples collected during the study period. SBA and CHROMagar detected 18 of 18 (100%) Acinetobacter and 11 of 11 (100%) MDR-A. baumannii. MacConkey agar detected 16 of 18 (89%) Acinetobacter and 10 of 11 (91%) MDR- A. baumannii while MacConkey agar with 6 μg/ml imipenem detected 9 of 11 (82%) MDR-A. baumannii. CHROMagar did not differentiate MDR- A. baumannii from non-MDR-A. baumannii. CHROMagar may be useful for rapid detection of patients with MDR-A. baumannii if improved upon to better select for MDR-A. baumannii.
本研究旨在评估 CHROMagar Acinetobacter 在检测住院患者监测培养物中的鲍曼不动杆菌时的性能，与绵羊血琼脂、MacConkey 琼脂和含 6μg/ml 亚胺培南的 MacConkey 琼脂进行比较。我们使用了 2009 年 12 月 7 日至 12 月 21 日期间入住马里兰大学医学中心 ICU 的患者的肛周拭子和痰液样本。样本按以下顺序接种在四种培养基上：（1）5%绵羊血琼脂（SBA）、（2）MacConkey 琼脂、（3）含 6μg/ml 亚胺培南的 MacConkey 琼脂和（4）CHROMagar Acinetobacter（CHROMagar）。SBA 是所有培养基比较的金标准。在研究期间共采集了 165 个样本。SBA 和 CHROMagar 检测到 18 株鲍曼不动杆菌和 11 株多药耐药鲍曼不动杆菌（MDR-A. baumannii），均为 100%。MacConkey 琼脂检测到 16 株鲍曼不动杆菌和 10 株 MDR-A. baumannii，为 89%，而含 6μg/ml 亚胺培南的 MacConkey 琼脂则检测到 11 株 MDR-A. baumannii 中的 9 株，为 82%。CHROMagar 无法区分 MDR-A. baumannii 和非 MDR-A. baumannii。如果进一步改进 CHROMagar 以更好地选择 MDR-A. baumannii，它可能对快速检测 MDR-A. baumannii 患者有用。
