阿霉素对肾上腺皮质线粒体孕烯醇酮生物合成的损害作用。

Impaired pregnenolone biosynthesis in adrenal cortex mitochondria by adriamycin.

作者信息

Cuéllar A, Díaz-Sánchez V, Altamarano A

机构信息

Department of Biochemistry, Instituto Nacional de Cardiología Ignacio Chávez, México City, Mexico.

出版信息

J Steroid Biochem. 1987 Oct;28(4):437-40. doi: 10.1016/0022-4731(87)91063-6.

DOI:10.1016/0022-4731(87)91063-6

PMID:3669664

Abstract

Adrenal cortex mitochondria isolated from mongrel dogs were incubated with cholesterol in the presence and absence of adriamycin (ADM). The capacity for cholesterol side chain cleavage was assessed by determining the pregnenolone yield. ADM behaves as an inhibitor of pregnenolone biosynthesis with a calculated IC50 of 110 microM. The inhibitory effect follows a dose-response relationship depending upon ADM concentration.

摘要

从杂种狗分离出的肾上腺皮质线粒体，在有和没有阿霉素（ADM）的情况下与胆固醇一起孵育。通过测定孕烯醇酮产量来评估胆固醇侧链裂解的能力。阿霉素表现为孕烯醇酮生物合成的抑制剂，计算得出的IC50为110微摩尔。抑制作用遵循剂量反应关系，具体取决于阿霉素的浓度。