State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, PR China; Institute of Theoretical Chemistry, College of Chemistry, Jilin University, No. 2 Liutiao Road, Changchun, 130023, PR China.
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, PR China; Institute of Theoretical Chemistry, College of Chemistry, Jilin University, No. 2 Liutiao Road, Changchun, 130023, PR China.
Anal Chim Acta. 2023 Feb 22;1243:340814. doi: 10.1016/j.aca.2023.340814. Epub 2023 Jan 10.
As a representative flavonoid, morin exhibits multi-biological activities, but its abuse endangers human health. Developing advanced technology for morin determination is urgently needed. In this study, a dual-responsive approach was reported for morin based on the complexing of carbon quantum dots (CQDs) and polyethyleneimine (PEI). The CQDs were fabricated via an improved hydrothermal method employing tyrosine and malic acid. Binding with PEI induced an 8-fold emission enhancement and a slight red-shift to 445 nm of CQDs because of the complexing of PEI and CQDs. Further morin introduction led to the blue emission (445 nm) quenching of CQDs-PEI and a yellow emission (560 nm) generation, which contributed a ratiometric fluorescence approach for morin determination between 2.0 and 32 μM, with a limit of detection (LOD) of 45 nM. Meanwhile, under sunlight the color of CQDs-PEI became yellow upon morin addition, which developed a colorimetric method for morin determination in a wide range between 2.0 and 100 μM (LOD = 69 nM). The developed dual-responsive method either displayed accurate results for morin in diluted human and bovine serum, being potential for actual sample analysis. Finally, a visual detection based on the smartphone was constructed and applied for the real-time determination of morin.
作为一种代表性的类黄酮，桑色素具有多种生物学活性，但过度使用会危害人类健康。因此，迫切需要开发先进的桑色素检测技术。本研究基于碳量子点（CQDs）与聚乙烯亚胺（PEI）的络合作用，报道了一种用于桑色素的双响应方法。通过采用酪氨酸和苹果酸的改进水热法制备了 CQDs。由于 PEI 与 CQDs 的络合作用，与 PEI 结合诱导 CQDs 的发射增强 8 倍，略微红移至 445nm。进一步引入桑色素导致 CQDs-PEI 的蓝色发射（445nm）猝灭和黄色发射（560nm）产生，这为 2.0 至 32μM 范围内的桑色素测定提供了一种比率荧光方法，检测限（LOD）为 45nM。同时，在阳光照射下，加入桑色素后 CQDs-PEI 的颜色变为黄色，这在 2.0 至 100μM 范围内发展了一种用于桑色素测定的比色方法（LOD=69nM）。所开发的双响应方法在稀释的人和牛血清中对桑色素的测定结果准确，具有实际样品分析的潜力。最后，构建了基于智能手机的可视化检测，并将其用于实时测定桑色素。
