聚亚胺基改性碳量子点的制备及其在甲氨蝶呤检测中的应用。
Preparation of Polyvinyl Imine Modified Carbon Quantum Dots and Their Application in Methotrexate Detection.
机构信息
Department of Food Science, Shanghai Business School, Shanghai 200235, China.
School of Materials and Chemical Engineering, Pingxiang University, Pingxiang 337055, China.
出版信息
Molecules. 2022 Aug 17;27(16):5254. doi: 10.3390/molecules27165254.
OBJECTIVE
A sensitive and selective fluorescence-detection platform based on carbon quantum dots (CQDs) was designed and developed for the determination of methotrexate (MTX), for the purpose of minimizing the possible toxic threat of MTX in clinics.
METHODS
The approach was prepared for the first time by a simple, hydrothermal method, making the synthesis and modification processes realized in one step using polyethyleneimine (PEI), and the proposed PEI-CQDs were obtained with high fluorescence quantum yield (38%).
RESULTS
MTX was found highly responsive and effective in quenching the fluorescence of the PEI-CQDs, due to a suggested fluorescence resonance energy transfer mechanism or inner-filter effect. The linear range of MTX was between 1 and 600 μmol/L under optimum conditions, with a detection limit (LOD) as low as 0.33 μmol/L. Furthermore, the fluorescent method was established for the MTX assay, and satisfactory results were acquired in real-sample determination. The average labeled quantity was 98.2%, and the average added standard recovery was 100.9%.
CONCLUSIONS
The proposed PEI-CQDs showed a remarkable potential for broad applications in biological molecule determination and environmental analysis.
目的
设计并开发了一种基于碳量子点（CQDs）的灵敏和选择性荧光检测平台，用于测定甲氨蝶呤（MTX），旨在最大限度地减少临床中 MTX 可能带来的毒性威胁。
方法
首次通过简单的水热法制备了该方法，使合成和修饰过程在一步中实现，使用聚乙烯亚胺（PEI），并获得了具有高荧光量子产率（38%）的提议的 PEI-CQDs。
结果
发现 MTX 非常有效地响应并猝灭了 PEI-CQDs 的荧光，这归因于建议的荧光共振能量转移机制或内滤效应。在最佳条件下，MTX 的线性范围在 1 至 600 μmol/L 之间，检测限（LOD）低至 0.33 μmol/L。此外，建立了用于 MTX 测定的荧光法，并在实际样品测定中获得了令人满意的结果。标记的平均量为 98.2%，平均加标回收率为 100.9%。
结论
提议的 PEI-CQDs 在生物分子测定和环境分析的广泛应用中显示出显著的潜力。
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