Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, 226001, China.
Department of Clinical Laboratory Shanghai East Hospital, Tongji University, Shanghai, 200123, China.
Mol Biotechnol. 2023 Sep;65(9):1528-1538. doi: 10.1007/s12033-023-00662-w. Epub 2023 Jan 25.
Lung cancer is one of the most dangerous malignant tumors to human health in the world. Previous researches have shown that cytoskeleton regulator RNA (CYTOR), a long noncoding RNA was involved in the occurrence and development of various types of cancer. The aim of this study is to investigate the clinical significance and biological function of CYTOR in lung cancer. Real-time quantitative PCR was applied to detect the expression of CYTOR. The proliferation of A549 and H1299 cells was analyzed by CCK8 assay. The luciferase reporter assay and RNA pull-down assay were used to reveal the interactions between CYTOR and its downstream targets. Western blot was used to detect the expression of high-mobility group protein B1 (HMGB1). Here we found CYTOR was upregulated in lung cancer tissues and cell lines. The proliferation of A549 and H1299 cells was inhibited after CYTOR silencing. In addition, CYTOR could directly interact with and negatively regulate miR-103a-3p, and miR-103a-3p inhibited cell proliferation by targeting HMGB1. The CYTOR/miR-103a-3p/HMGB1 axis promoted lung cancer cell proliferation. CYTOR sponges miR-103a-3p to promote the proliferation of lung cancer cells through HMGB1. The CYTOR/miR-103a-3p/HMGB1 axis plays a critical role in the progression of lung cancer.
肺癌是世界上对人类健康最危险的恶性肿瘤之一。先前的研究表明，细胞骨架调节 RNA（CYTOR），一种长非编码 RNA，参与了各种类型癌症的发生和发展。本研究旨在探讨 CYTOR 在肺癌中的临床意义和生物学功能。实时定量 PCR 用于检测 CYTOR 的表达。CCK8 assay 分析 A549 和 H1299 细胞的增殖。荧光素酶报告实验和 RNA 下拉实验用于揭示 CYTOR 与其下游靶标的相互作用。Western blot 用于检测高迁移率族蛋白 B1（HMGB1）的表达。结果发现 CYTOR 在肺癌组织和细胞系中上调。沉默 CYTOR 后，A549 和 H1299 细胞的增殖受到抑制。此外，CYTOR 可以直接相互作用并负调控 miR-103a-3p，miR-103a-3p 通过靶向 HMGB1 抑制细胞增殖。CYTOR/miR-103a-3p/HMGB1 轴促进肺癌细胞增殖。CYTOR 通过 HMGB1 海绵吸附 miR-103a-3p 促进肺癌细胞增殖。CYTOR/miR-103a-3p/HMGB1 轴在肺癌的进展中起着关键作用。
