Department of Clinical and Chemical Pathology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Department of Obstetrics and Gynecology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
BMC Cancer. 2023 Jan 25;23(1):89. doi: 10.1186/s12885-023-10549-3.
Ovarian cancer is a leading cause of female mortality. Epigenetic changes occur in early stages of carcinogenesis and represent a marker for cancer diagnosis. Protocadherin 17 (PCDH17) is a tumor suppressor gene involved in cell adhesion and apoptosis. The methylation of PCDH17 gene promoter has been described in several cancers including ovarian cancer. The aim of the study was to compare the methylation status of PCDH17 gene promoter between females diagnosed with epithelial ovarian cancer and a control group composed of normal and benign ovarian lesions.
Fifty female subjects were included in our study (25 ovarian cancer patients and 25 controls). DNA was extracted from Formalin-Fixed Paraffin-Embedded (FFPE) tissues of the subjects. Methylation levels for six CpG sites in the PCDH17 gene promoter were assessed by pyrosequencing.
The methylation levels at five out of six sites were significantly higher in females with epithelial ovarian cancer compared to the control group. Moreover, the same applies for the mean methylation level with p value 0.018.
Methylation of PCDH17 gene promoter plays a role in ovarian carcinogenesis and can be used for diagnosis and early detection.
卵巢癌是女性死亡的主要原因之一。在癌变的早期就会发生表观遗传改变，这是癌症诊断的一个标志物。原钙黏蛋白 17（PCDH17）是一种参与细胞黏附和细胞凋亡的肿瘤抑制基因。PCDH17 基因启动子的甲基化已在包括卵巢癌在内的多种癌症中被描述。本研究旨在比较上皮性卵巢癌患者和由正常和良性卵巢病变组成的对照组女性中 PCDH17 基因启动子的甲基化状态。
本研究纳入了 50 名女性受试者（25 名卵巢癌患者和 25 名对照组）。从受试者的福尔马林固定石蜡包埋（FFPE）组织中提取 DNA。通过焦磷酸测序评估 PCDH17 基因启动子中六个 CpG 位点的甲基化水平。
与对照组相比，上皮性卵巢癌女性中六个位点中的五个位点的甲基化水平显著升高。同样，平均甲基化水平的 p 值也为 0.018。
PCDH17 基因启动子的甲基化在卵巢癌发生中起作用，可用于诊断和早期检测。
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