Nematode Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh 202002, India.
J Helminthol. 2023 Jan 26;97:e11. doi: 10.1017/S0022149X2200089X.
A new isolate of (Bütschli, ) Dougherty, is described and illustrated with morphological and molecular data. The phylogenetic analysis based on the D2/D3 segment of 28S rDNA using the Bayesian inference method, revealed monophyly of the genus as the subordinate taxa clustered in one clade. The clade further divided into two subclades representing the group and group with 100% posterior probability values. However, GenBank sequences of several species constituting the -group, showed high similarity and very little genetic divergence (98-99%) of up to 4-5 bases. In order to ascertain the status of those isolates, detailed morphological comparison is provided along with a pictorial key. A sequence-based phylogeography of haplogroups of using the median-joining network method, was also inferred. The results suggested the need for morphological validation of a species before its sequences are deposited in GenBank.
描述并图示了一个新的 （Bütschli，）Dougherty 的分离株，并提供了形态学和分子数据。基于 D2/D3 片段的 28S rDNA 的贝叶斯推断方法的系统发育分析表明，属的单系性是下属分类群聚集在一个分支中。该分支进一步分为两个亚分支，代表 和 组，后验概率值为 100%。然而，构成 - 组的几个物种的 GenBank 序列显示出高度相似性和非常小的遗传差异（98-99%），多达 4-5 个碱基。为了确定这些分离株的地位，提供了详细的形态比较以及图片式关键。还使用中位数连接网络方法推断了 的单倍型组的基于序列的系统地理学。结果表明，在将序列存入 GenBank 之前，需要对物种进行形态学验证。
