Ekman S
Department of Biochemistry and Pharmacy, Abo Akademi, Turku, Finland.
Lipids. 1987 Sep;22(9):657-63. doi: 10.1007/BF02533945.
A new technique for preparing microemulsion particles of well-defined sizes and compositions is presented. Utilization of these microemulsions is advocated as lipoprotein models in studies of lipid transport and metabolism, rather than the currently used phospholipid-cholesterol vesicles. The emulsion particles consisted of egg phosphatidylcholine and cholesterol as surface lipids and cholesteryl oleate as core lipid. They were prepared by a combined injection and sonication technique and size-separated by a two-step procedure of gel filtration chromatography and density gradient centrifugation. By varying the ratios of core and surface material, particles covering a size range of 20-200 nm in diameter could be produced. The adequacy of these microemulsions as lipoprotein models was tested by studying the transfer of [3H]cholesterol and [14C]cholesterol oleate from the particles to cultured human fibroblasts. Up to a particle size of 100 nm, there was a slight increase of [3H]cholesterol transfer. The transfer of [14C]cholesteryl oleate was very slow, yet measurable. Studies of the exchangeability of cholesterol between the microemulsion core and surface phases indicated that all cholesterol can be transferred from microemulsions to cultured cells as a single pool.
本文介绍了一种制备尺寸和组成明确的微乳液颗粒的新技术。提倡将这些微乳液用作脂蛋白模型，用于脂质转运和代谢研究，而不是目前使用的磷脂 - 胆固醇囊泡。乳液颗粒由作为表面脂质的鸡蛋磷脂酰胆碱和胆固醇以及作为核心脂质的胆固醇油酸酯组成。它们通过联合注射和超声处理技术制备，并通过凝胶过滤色谱和密度梯度离心的两步程序进行尺寸分离。通过改变核心和表面材料的比例，可以生产出直径范围为20 - 200 nm的颗粒。通过研究[3H]胆固醇和[14C]胆固醇油酸酯从颗粒向培养的人成纤维细胞的转移，测试了这些微乳液作为脂蛋白模型的适用性。在颗粒尺寸达到100 nm之前，[3H]胆固醇转移略有增加。[14C]胆固醇油酸酯的转移非常缓慢，但仍可测量。微乳液核心和表面相之间胆固醇交换性的研究表明，所有胆固醇都可以作为一个单一库从微乳液转移到培养细胞中。
