Nishio Joichiro, Takahashi Yoshiaki, Kasahara Masahiro, Takeda Yoichi, Kikuma Takashi
Department of Biotechnology, College of Life Sciences, Ritsumeikan University, Kusatsu, Japan.
FEBS Lett. 2023 Mar;597(5):608-617. doi: 10.1002/1873-3468.14589. Epub 2023 Feb 2.
Autophagy is classified into nonselective and selective autophagy, depending on the specificity of substrate degradation. In the filamentous fungus Aspergillus oryzae, selective autophagy, which includes pexophagy and mitophagy, has been observed. However, the molecular mechanism underlying selective autophagy in filamentous fungi remains unclear. Here, we identified a novel protein that interacts with the autophagy-related protein Atg8 in A. oryzae, named AoAtg8-interacting protein A (AeiA). AeiA was localized to AoAtg8-positive autophagic membrane structures and peroxisomes. Moreover, peroxisomal trafficking into the vacuole was reduced in AeiA disruptants. Taken together, AeiA is a novel selective autophagy-related protein that contributes to pexophagy in A. oryzae.
自噬根据底物降解的特异性分为非选择性自噬和选择性自噬。在丝状真菌米曲霉中，已经观察到包括过氧化物酶体自噬和线粒体自噬在内的选择性自噬。然而，丝状真菌中选择性自噬的分子机制仍不清楚。在这里，我们鉴定了一种与米曲霉中自噬相关蛋白Atg8相互作用的新蛋白，命名为AoAtg8相互作用蛋白A（AeiA）。AeiA定位于AoAtg8阳性的自噬膜结构和过氧化物酶体。此外，在AeiA缺失突变体中，过氧化物酶体向液泡的运输减少。综上所述，AeiA是一种新的选择性自噬相关蛋白，它有助于米曲霉中的过氧化物酶体自噬。
