State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Jiangxi General Institute of Testing and Certification, Nanchang 330029, China.
Food Chem. 2023 Jun 15;411:135511. doi: 10.1016/j.foodchem.2023.135511. Epub 2023 Jan 18.
As kanamycin (KAN) residue in animal products is harmful to consumers, a rapid and sensitive method for KAN detection needs to be established. KAN monoclonal antibody (KAN-mAb, 1D11) with the half maximal inhibitory concentration of 1.16 ng/mL was prepared in this study. A one-pot method was used to synthesize Fe-chelated polydopamine nanospheres (Fe@PDANs) with excellent characteristics of strong light absorption. The novel label of Fe@PDANs and KAN-mAb was used to develop a lateral flow immunoassay (LFIA) for the sensitive detection of KAN. The limit of detection of the Fe@PDANs-based LFIA (Fe@PDANs-LFIA) for KAN was 0.0191 ng/mL, which was 2.75 times lower than PDANs-based LFIA. Furthermore, the Fe@PDANs-LFIA was successfully applied to detect KAN in pork, milk, and honey samples, with recoveries ranging from 93.75% to 113.80% (coefficient of variation < 10%). Therefore, Fe@PDANs have potential for the detection of analytes in LFIA.
由于动物产品中的卡那霉素（KAN）残留对消费者有害，因此需要建立一种快速、灵敏的 KAN 检测方法。本研究制备了半抑制浓度为 1.16ng/mL 的 KAN 单克隆抗体（KAN-mAb，1D11）。采用一锅法合成了具有强吸光特性的 Fe 螯合聚多巴胺纳米球（Fe@PDANs）。新型 Fe@PDANs 和 KAN-mAb 标记物用于开发用于灵敏检测 KAN 的侧向流动免疫分析（LFIA）。基于 Fe@PDANs 的 LFIA（Fe@PDANs-LFIA）检测 KAN 的检出限为 0.0191ng/mL，比 PDANs 基 LFIA 低 2.75 倍。此外，Fe@PDANs-LFIA 成功应用于猪肉、牛奶和蜂蜜样品中 KAN 的检测，回收率在 93.75%至 113.80%之间（变异系数 < 10%）。因此，Fe@PDANs 在 LFIA 中检测分析物具有潜力。
