Ribeiro Rodrigo M, Ribeiro Debora da Silva Freitas, Cota Leticia Oliveira, Carvalho Armando Mattos, Gobesso Alexandre Augusto de Oliveira, Faleiros Rafael R
Centro Universitário de Mineiros, Department of Veterinary Medicine, Mineiros, Goiás, Brazil.
Centro Universitário de Mineiros, Department of Veterinary Medicine, Mineiros, Goiás, Brazil.
J Equine Vet Sci. 2023 Mar;122:104230. doi: 10.1016/j.jevs.2023.104230. Epub 2023 Jan 24.
This study aimed to compare the use of enzymatic colorimetry and Friedewald's formula for the determination of LDL in horses. A total of 260 samples were used. Direct analysis was performed to determine low-density lipoprotein (LDL), high-density lipoprotein (HDL) cholesterol, triglycerides (TG), and total cholesterol (TC). The LDL level was calculated using the Friedewald equation (LDL= TC-HDL-TG/5). The correlations between the direct LDL analysis and the LDL calculated using the Friedewald formula were determined by Pearson's tests, and the coefficient of determination was also obtained by linear regression using SAS software (P<.05) and the kappa value. The mean value (± standard deviation) of the LDL was 22.12 (±10.34) mg/dL, and that of the result obtained by the Friedewald formula was 19.94 (±19.13) mg/dL. The correlation between the two variants analyzed in this experiment was significant, with a value of P < .001, and values of r = 0.688 and R = 0.4893. These results are relevant, given that recent studies have demonstrated a correlation between LDL plasma values and equine metabolic syndrome. Previous studies have reported discrepancies between the data obtained using Friedewald's method and the LDL-c values directly determined in humans and animals. The results of the present study suggest that the Friedewald method can be used to estimate the LDL plasma concentration in horses. Nevertheless, the coefficient of determination was not found to be adequate to recommend the Friedewald formula as a replacement for the enzymatic colorimetric method in determining LDL in horses.
本研究旨在比较酶比色法和弗里德瓦尔德公式在测定马低密度脂蛋白（LDL）中的应用。共使用了260份样本。进行直接分析以测定低密度脂蛋白（LDL）、高密度脂蛋白（HDL）胆固醇、甘油三酯（TG）和总胆固醇（TC）。使用弗里德瓦尔德方程（LDL = TC - HDL - TG/5）计算LDL水平。通过皮尔逊检验确定直接LDL分析与使用弗里德瓦尔德公式计算的LDL之间的相关性，并使用SAS软件通过线性回归获得决定系数（P<.05）和kappa值。LDL的平均值（±标准差）为22.12（±10.34）mg/dL，弗里德瓦尔德公式得出的结果平均值为19.94（±19.13）mg/dL。本实验分析的两个变量之间的相关性显著，P值<0.001，r值 = 0.688，R值 = 0.4893。鉴于最近的研究表明LDL血浆值与马代谢综合征之间存在相关性，这些结果具有重要意义。先前的研究报告了使用弗里德瓦尔德方法获得的数据与在人和动物中直接测定的LDL-c值之间存在差异。本研究结果表明，弗里德瓦尔德方法可用于估计马的LDL血浆浓度。然而，未发现决定系数足以推荐弗里德瓦尔德公式替代酶比色法来测定马的LDL。
