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通过蛋白激酶C对受肿瘤启动子和有丝分裂原调控的基因序列进行分子克隆。

Molecular cloning of gene sequences regulated by tumor promoters and mitogens through protein kinase C.

作者信息

Johnson M D, Housey G M, Kirschmeier P T, Weinstein I B

机构信息

Cancer Center, College of Physicians and Surgeons, Columbia University, New York, New York 10032.

出版信息

Mol Cell Biol. 1987 Aug;7(8):2821-9. doi: 10.1128/mcb.7.8.2821-2829.1987.

DOI:10.1128/mcb.7.8.2821-2829.1987
PMID:3670294
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC367899/
Abstract

cDNA clones representing genes whose expression is modulated by treatment with mitogens and tumor promoters were isolated and characterized. TPA-S1 corresponds to an mRNA species whose abundance was increased markedly within 1 h of exposure to the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA), and TPA-R1 represents an mRNA that was decreased in TPA-treated cells. The induction of TPA-S1 was blocked by actinomycin D but was not affected by cycloheximide, and it was specific for phorbol esters with tumor-promoting activity. The role of protein kinase C in the induction of TPA-S1 is supported by the following lines of evidence. (i) Agents that activated protein kinase C (TPA, platelet-derived growth factor, and diacylglycerol) also increased TPA-S1 mRNA levels. (ii) A potent PKC inhibitor blocked the induction of TPA-S1. (iii) Down-regulation of PKC activity, by treatment of cells with TPA for 24 h, resulted in a loss of responsiveness to TPA-S1 induction by subsequent TPA treatment. DNA sequence analysis of TPA-S1 predicts a cysteine-rich, secreted protein with a molecular weight of 22.6 X 10(3) that exhibits homology with sequences representing a protein with human erythroid-potentiating activity and protease inhibitory activity.

摘要

分离并鉴定了代表其表达受促细胞分裂剂和肿瘤启动子处理调节的基因的cDNA克隆。TPA-S1对应于一种mRNA种类，其丰度在暴露于肿瘤启动子12-O-十四烷酰佛波醇-13-乙酸酯（TPA）后1小时内显著增加，而TPA-R1代表在TPA处理的细胞中减少的一种mRNA。TPA-S1的诱导被放线菌素D阻断，但不受环己酰亚胺影响，并且它对具有肿瘤促进活性的佛波酯具有特异性。蛋白激酶C在TPA-S1诱导中的作用得到以下几方面证据的支持。（i）激活蛋白激酶C的试剂（TPA、血小板衍生生长因子和二酰基甘油）也增加了TPA-S1 mRNA水平。（ii）一种有效的PKC抑制剂阻断了TPA-S1的诱导。（iii）通过用TPA处理细胞24小时来下调PKC活性，导致随后用TPA处理时对TPA-S1诱导的反应性丧失。TPA-S1的DNA序列分析预测了一种富含半胱氨酸的分泌蛋白，分子量为22.6×10³，与代表具有人红细胞增强活性和蛋白酶抑制活性的蛋白的序列具有同源性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/32f80ab79a73/molcellb00080-0185-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/036791e33215/molcellb00080-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/050504404a3a/molcellb00080-0181-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/20bd14742f9f/molcellb00080-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/ba37084fe944/molcellb00080-0182-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/f2fcfabb1ce0/molcellb00080-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/227ff67cfbee/molcellb00080-0183-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/b10789368b35/molcellb00080-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/32f80ab79a73/molcellb00080-0185-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/036791e33215/molcellb00080-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/050504404a3a/molcellb00080-0181-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/20bd14742f9f/molcellb00080-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/ba37084fe944/molcellb00080-0182-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/f2fcfabb1ce0/molcellb00080-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/227ff67cfbee/molcellb00080-0183-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/b10789368b35/molcellb00080-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/424c/367899/32f80ab79a73/molcellb00080-0185-a.jpg

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本文引用的文献

1
Human platelet-derived growth factor. Purification and resolution into two active protein fractions.人血小板衍生生长因子。纯化并分离为两个活性蛋白组分。
J Biol Chem. 1981 Sep 10;256(17):8896-9.
2
Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.肿瘤促进剂佛波酯对钙激活的、磷脂依赖性蛋白激酶的直接激活作用。
J Biol Chem. 1982 Jul 10;257(13):7847-51.
3
Synthesis and processing of asparagine-linked oligosaccharides.天冬酰胺连接寡糖的合成与加工
J Cell Biol. 1997 Jul 14;138(1):203-13. doi: 10.1083/jcb.138.1.203.
4
Cross-linking of T-cell receptors on double-positive thymocytes induces a cytokine-mediated stromal activation process linked to cell death.双阳性胸腺细胞上T细胞受体的交联诱导了一个与细胞死亡相关的细胞因子介导的基质激活过程。
EMBO J. 1996 Nov 1;15(21):5876-87.
5
Role of protein kinase C in growth stimulation of primary mouse colonic epithelial cells.蛋白激酶C在原代小鼠结肠上皮细胞生长刺激中的作用。
In Vitro Cell Dev Biol Anim. 1995 Nov;31(10):790-5. doi: 10.1007/BF02634121.
6
Tumor promoter-inducible genes are differentially expressed in the developing mouse.肿瘤启动子诱导基因在发育中的小鼠中差异表达。
Mol Cell Biol. 1988 Oct;8(10):4570-2. doi: 10.1128/mcb.8.10.4570-4572.1988.
7
Induction of a mitogen-responsive gene after expression of the Ha-ras oncogene in NIH 3T3 fibroblasts.在NIH 3T3成纤维细胞中表达Ha-ras癌基因后诱导有丝分裂原反应基因。
Mol Cell Biol. 1989 Nov;9(11):5207-14. doi: 10.1128/mcb.9.11.5207-5214.1989.
8
Role of protein kinase C in regulation of gene expression and relevance to tumor promotion.蛋白激酶C在基因表达调控中的作用及其与肿瘤促进的相关性。
Environ Health Perspect. 1987 Dec;76:89-95. doi: 10.1289/ehp.877689.
9
Growth factors and oncogenes in human gastrointestinal carcinomas.人类胃肠道癌中的生长因子与癌基因
J Cancer Res Clin Oncol. 1990;116(2):121-31. doi: 10.1007/BF01612665.
10
Angiotensin II induces secretion of plasminogen activator inhibitor 1 and a tissue metalloprotease inhibitor-related protein from rat brain astrocytes.血管紧张素II诱导大鼠脑星形胶质细胞分泌纤溶酶原激活物抑制剂1和一种组织金属蛋白酶抑制剂相关蛋白。
Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1928-32. doi: 10.1073/pnas.88.5.1928.
Annu Rev Biochem. 1981;50:555-83. doi: 10.1146/annurev.bi.50.070181.003011.
4
In vitro studies on the mode of action of the phorbol esters, potent tumor promoters, part 2.佛波酯（一种有效的肿瘤促进剂）作用模式的体外研究，第2部分。
Crit Rev Toxicol. 1981 Jun;8(3):199-234. doi: 10.3109/10408448109109658.
5
Epidermal growth factor rapidly stimulates prolactin gene transcription.表皮生长因子迅速刺激催乳素基因转录。
Nature. 1982 Nov 11;300(5888):192-4. doi: 10.1038/300192a0.
6
Molecular cloning of gene sequences regulated by platelet-derived growth factor.血小板衍生生长因子调控的基因序列的分子克隆
Cell. 1983 Jul;33(3):939-47. doi: 10.1016/0092-8674(83)90037-5.
7
Structure and expression of a cloned cDNA for mouse interferon-beta.小鼠β干扰素克隆cDNA的结构与表达
J Biol Chem. 1983 Aug 10;258(15):9522-9.
8
Tumor-promoting phorbol esters inhibit procollagen synthesis at a pretranslational level in JB-6 mouse epidermal cells.促肿瘤佛波酯在翻译前水平抑制JB-6小鼠表皮细胞中的前胶原合成。
Mol Cell Biol. 1983 Aug;3(8):1527-32. doi: 10.1128/mcb.3.8.1527-1532.1983.
9
Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor.淋巴细胞有丝分裂原和血小板衍生生长因子对c-myc基因的细胞特异性调控。
Cell. 1983 Dec;35(3 Pt 2):603-10. doi: 10.1016/0092-8674(83)90092-2.
10
Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate.1,4,5-三磷酸肌醇促使胰腺腺泡细胞非线粒体胞内钙库释放钙离子。
Nature. 1983;306(5938):67-9. doi: 10.1038/306067a0.