Klock G, Strähle U, Schütz G
Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelber.
Nature. 1987;329(6141):734-6. doi: 10.1038/329734a0.
DNA sequences recognized by the glucocorticoid receptor are termed glucocorticoid-responsive elements because of their stimulatory effect on transcription. An oligonucleotide of 15 base pairs having partial or perfect symmetry is necessary for glucocorticoid induction and this same oligonucleotide is surprisingly also recognized by the progesterone receptor. Here we define a palindromic sequence of 15 base pairs, modelled after a sequence element shared by the vitellogenin genes of frog and chicken, which confers oestrogen inducibility on a heterologous promoter and can be converted into a glucocorticoid-responsive element by substitution of one or two bases at homologous positions in the palindrome. Considered with the observation that the DNA-binding domains of steroid receptors are closely related, this finding demonstrates that the steroid-responsive elements constitute a family of related DNA sequences.
由于对转录有刺激作用，被糖皮质激素受体识别的DNA序列被称为糖皮质激素反应元件。具有部分或完全对称性的15个碱基对的寡核苷酸对于糖皮质激素诱导是必需的，而且令人惊讶的是，这个相同的寡核苷酸也被孕激素受体识别。在这里，我们定义了一个15个碱基对的回文序列，它是以青蛙和鸡的卵黄蛋白原基因共有的一个序列元件为模型的，该序列赋予异源启动子雌激素诱导性，并且通过在回文中同源位置替换一个或两个碱基可以将其转化为糖皮质激素反应元件。结合类固醇受体的DNA结合结构域密切相关这一观察结果，这一发现表明类固醇反应元件构成了一个相关DNA序列家族。
