Choi Justin, Bordeaux Zachary A, Braun Gabriella, Davis Cole, Parthasarathy Varsha, Deng Junwen, Taylor Mathew T, Kambala Anusha, Cornman Hannah, Oladipo Olusola, Alphonse Martin P, West Cameron E, Kwatra Shawn G, Kwatra Madan M
Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Department of Anesthesiology, Duke University School of Medicine, Durham, North Carolina, USA.
JID Innov. 2022 Sep 29;3(1):100107. doi: 10.1016/j.xjidi.2022.100164. eCollection 2023 Jan.
UV irradiation is commonly used in murine models of skin cancers. Despite the popularity of using UVB rays to model photocarcinogenesis in animals, there is a lack of standardization in the secondary enclosures used to administer radiation. An appraisal of the literature also shows a general lack of details regarding the materials and procedures utilized in the fabrication of such enclosures. We present in this study a detailed overview of the construction of a UVB exposure chamber that successfully induces lesions in hairless mice. A standardized protocol for producing a UVB enclosure may reduce methodological variation in future studies seeking to investigate photocarcinogenesis in animals.
紫外线照射常用于皮肤癌的小鼠模型。尽管使用紫外线B射线在动物中模拟光致癌作用很普遍，但用于进行辐射的二级围护结构缺乏标准化。对文献的评估还表明，对于此类围护结构制造中所使用的材料和程序，普遍缺乏详细信息。在本研究中，我们详细概述了一个能成功诱导无毛小鼠产生损伤的紫外线B照射室的构建。制定一个生产紫外线B围护结构的标准化方案，可能会减少未来旨在研究动物光致癌作用的研究中的方法学差异。
Zotero 插件