Gratton A, Hoffer B J, Freedman R
Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262.
Neuropharmacology. 1987 Sep;26(9):1275-83. doi: 10.1016/0028-3908(87)90087-6.
The effects of local applications of phencyclidine (PCP) and dopamine (DA) on neurons of the medial prefrontal cortex were investigated using single unit recording techniques. The activity of the majority of cells in the deeper layers of the medial prefrontal cortex was depressed by both phencyclidine and DA, whereas increases, as well as decreases, in the firing rates were observed in cells located in the superficial cortical layers. The stereospecificity of the responses of deeper cells to phencyclidine was demonstrated using the enantiomers of 1-(-1-phenylcyclohexyl)-3-methylpiperidine (PCMP). Phencyclidine was found to be 1.5 times more potent than (+) PCMP and 3 times more potent than (-) PCMP. Finally, the DA receptor antagonist fluphenazine, blocked the phencyclidine-elicited depressions of unit activity in the deep prefrontal cortex. Taken together, the data indicate that the DA-like effects of phencyclidine on neurons of the medial prefrontal cortex are mediated by DA receptors and provide pharmacological support for the idea that psychomotor stimulant drugs have specific actions on targets of the ventral tegmental area (A10) dopamine system.
运用单单位记录技术，研究了局部应用苯环己哌啶（PCP）和多巴胺（DA）对内侧前额叶皮质神经元的影响。内侧前额叶皮质深层大多数细胞的活性受到PCP和DA的抑制，而位于皮质浅层的细胞则观察到放电频率增加以及减少的情况。使用1-（-1-苯基环己基）-3-甲基哌啶（PCMP）的对映体证明了深层细胞对PCP反应的立体特异性。发现PCP的效力比（+）PCMP高1.5倍，比（-）PCMP高3倍。最后，DA受体拮抗剂氟奋乃静阻断了PCP引起的前额叶皮质深层单位活性的抑制。综上所述，数据表明PCP对内侧前额叶皮质神经元的类DA样作用是由DA受体介导的，并为精神运动兴奋剂药物对腹侧被盖区（A10）多巴胺系统靶点具有特定作用这一观点提供了药理学支持。
