通过外部符合计数法对大鼠脑内[18F]氟脱氧葡萄糖速率常数进行体内测量。

In vivo measurement of [18F]fluorodeoxyglucose rate constants in rat brain by external coincidence counting.

作者信息

Redies C, Matsuda H, Diksic M, Meyer E, Yamamoto Y L

机构信息

Cone Laboratory for Neurosurgical Research, Montreal Neurological Institute and Hospital, Quebec, Canada.

出版信息

Neuroscience. 1987 Aug;22(2):593-9. doi: 10.1016/0306-4522(87)90356-3.

DOI:10.1016/0306-4522(87)90356-3

PMID:3670599

Abstract

The operational equation for the double-label deoxyglucose method described in the following paper requires the knowledge of the rate constants for transfer of fluorodeoxyglucose across the blood-brain barrier (K1* and K2*), and those for phosphorylation of fluorodeoxyglucose (K3*) and dephosphorylation of fluorodeoxyglucose-6-phosphate (k4*). These rate constants were determined in anesthetized rats by external coincidence counting. Radioactivity in parietal brain was measured for a 110 min experimental period after a bolus injection of 18F-labeled fluorodeoxyglucose. Apparent rate constants were obtained by fitting the resulting tissue radioactivity curves to the tissue radioactivity function of the deoxyglucose model modified to take into account the dephosphorylation of fluorodeoxyglucose-6-phosphate. The apparent fluorodeoxyglucose rate constants in rat brain are K1* = 0.195 ml g-1 min-1, k2* = 0.379 min-1, k3* = 0.088 min-1, and k4* = 0.009 min-1.

摘要

以下论文中描述的双标记脱氧葡萄糖法的运算方程需要了解氟代脱氧葡萄糖穿过血脑屏障的速率常数（K1和K2），以及氟代脱氧葡萄糖磷酸化（K3*）和氟代脱氧葡萄糖-6-磷酸去磷酸化（k4*）的速率常数。这些速率常数是通过外部符合计数在麻醉大鼠中测定的。在静脉推注18F标记的氟代脱氧葡萄糖后，在110分钟的实验期内测量顶叶脑的放射性。通过将所得组织放射性曲线拟合到考虑了氟代脱氧葡萄糖-6-磷酸去磷酸化的脱氧葡萄糖模型的组织放射性函数，获得表观速率常数。大鼠脑中的表观氟代脱氧葡萄糖速率常数为K1* = 0.195 ml g-1 min-1，k2* = 0.379 min-1，k3* = 0.088 min-1，k4* = 0.009 min-1。