Redies C, Diksic M, Evans A C, Gjedde A, Yamamoto Y L
Cone Laboratory for Neurosurgical Research, Montreal Neurological Institute and Hospital, Canada.
Neuroscience. 1987 Aug;22(2):601-19. doi: 10.1016/0306-4522(87)90357-5.
A new double-label autoradiographic glucose analog method for the sequential measurement of altered regional cerebral metabolic rates for glucose in the same animal is presented. This method is based on the sequential injection of two boluses of glucose tracer labeled with two different isotopes (short-lived 18F and long-lived 3H, respectively). An operational equation is derived which allows the determination of glucose utilization for the time period before the injection of the second tracer; this equation corrects for accumulation and loss of the first tracer from the metabolic pool occurring after the injection of the second tracer. An error analysis of this operational equation is performed. The double-label deoxyglucose method is validated in the primary somatosensory ("barrel") cortex of the anesthetized rat. Two different rows of whiskers were stimulated sequentially in each rat; the two periods of stimulation were each preceded by an injection of glucose tracer. After decapitation, dried brain slices were first exposed, in direct contact, to standard X-ray film and then to uncoated, "tritium-sensitive" film. Results show that the double-label deoxyglucose method proposed in this paper allows the quantification and complete separation of glucose utilization patterns elicited by two different stimulations sequentially applied in the same animal. The double-label deoxyglucose is of potential usefulness in sensory physiology since it makes possible the separate mapping of regional cerebral glucose utilization patterns elicited by two sequentially applied sensory stimulations in the same animal. The method allows the quantification of a step-like change in regional cerebral glucose utilization in the same animal. It could be used to study the cerebral metabolic effects induced by neuropharmacological agents or surgical interventions applied during the experiment. Using each animal as its own control eliminates intersubject variability. Thus experimental cost and effort can be saved, and the reliability of the results obtained can be increased.
本文介绍了一种新的双标记放射自显影葡萄糖类似物方法，用于在同一动物中连续测量局部脑葡萄糖代谢率的变化。该方法基于先后注射两剂分别用两种不同同位素（短寿命的18F和长寿命的3H）标记的葡萄糖示踪剂。推导出一个运算方程，可用于确定注射第二种示踪剂之前时间段内的葡萄糖利用率；该方程校正了注射第二种示踪剂后第一种示踪剂在代谢库中的积累和损失。对该运算方程进行了误差分析。在麻醉大鼠的初级体感（“桶状”）皮层中验证了双标记脱氧葡萄糖法。在每只大鼠中依次刺激两排不同的胡须；在每次刺激期之前均注射葡萄糖示踪剂。断头后，将干燥的脑片首先直接与标准X射线胶片接触曝光，然后再与未涂布的“对氚敏感”胶片接触曝光。结果表明，本文提出的双标记脱氧葡萄糖法能够对同一动物中先后施加的两种不同刺激所引发的葡萄糖利用模式进行定量和完全分离。双标记脱氧葡萄糖在感觉生理学中具有潜在用途，因为它能够对同一动物中先后施加的两种感觉刺激所引发的局部脑葡萄糖利用模式进行单独映射。该方法能够对同一动物中局部脑葡萄糖利用的阶梯状变化进行定量。它可用于研究实验过程中应用神经药理学药物或手术干预所诱导的脑代谢效应。以每只动物自身作为对照可消除个体间差异。因此可以节省实验成本和工作量，并提高所得结果的可靠性。
