靶向二代测序技术在直接从支气管肺泡灌洗液体外DNA诊断结核分枝杆菌及一线药物耐药性中的应用 - Suppr

Application of targeted next generation sequencing technology in the diagnosis of Mycobacterium Tuberculosis and first line drugs resistance directly from cell-free DNA of bronchoalveolar lavage fluid.

作者信息

Wu Xiaocui, Liang Ruobing, Xiao Yanqun, Liu Huan, Zhang Ye, Jiang Yue, Liu Mengdi, Tang Jianzhong, Wang Wei, Li Wei, Hu Long, Wang Aihua, Yu Fangyou, Xia Han

机构信息

Department of Clinical Laboratory, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China.

Department of Scientific Affaires, Hugobiotech Co., Ltd., Beijing, China.

出版信息

J Infect. 2023 Apr;86(4):399-401. doi: 10.1016/j.jinf.2023.01.031. Epub 2023 Jan 24.

DOI:10.1016/j.jinf.2023.01.031

PMID:36706961

Abstract

摘要

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Application of targeted next generation sequencing technology in the diagnosis of Mycobacterium Tuberculosis and first line drugs resistance directly from cell-free DNA of bronchoalveolar lavage fluid.

作者信息

Wu Xiaocui, Liang Ruobing, Xiao Yanqun, Liu Huan, Zhang Ye, Jiang Yue, Liu Mengdi, Tang Jianzhong, Wang Wei, Li Wei, Hu Long, Wang Aihua, Yu Fangyou, Xia Han

机构信息

Department of Clinical Laboratory, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China.

Department of Scientific Affaires, Hugobiotech Co., Ltd., Beijing, China.

出版信息

J Infect. 2023 Apr;86(4):399-401. doi: 10.1016/j.jinf.2023.01.031. Epub 2023 Jan 24.

DOI:10.1016/j.jinf.2023.01.031

PMID:36706961

Abstract

摘要

相似文献

J Infect. 2023 Apr;86(4):399-401. doi: 10.1016/j.jinf.2023.01.031. Epub 2023 Jan 24.

The value of metagenomic next-generation sequencing for the diagnosis of pulmonary tuberculosis using bronchoalveolar lavage fluid.

Lab Med. 2024 Jan 6;55(1):96-102. doi: 10.1093/labmed/lmad041.

Tuberculosis Diagnosis by Metagenomic Next-generation Sequencing on Bronchoalveolar Lavage Fluid: a cross-sectional analysis.

Int J Infect Dis. 2021 Mar;104:50-57. doi: 10.1016/j.ijid.2020.12.063. Epub 2020 Dec 24.

A preliminary evaluation of targeted nanopore sequencing technology for the detection of in bronchoalveolar lavage fluid specimens.

Front Cell Infect Microbiol. 2023 Nov 10;13:1107990. doi: 10.3389/fcimb.2023.1107990. eCollection 2023.

Diagnostic value of metagenomic next-generation sequencing of bronchoalveolar lavage fluid for the diagnosis of suspected pneumonia in immunocompromised patients.

BMC Infect Dis. 2022 Apr 29;22(1):416. doi: 10.1186/s12879-022-07381-8.

The application of nanopore targeted sequencing for pathogen diagnosis in bronchoalveolar lavage fluid of patients with pneumonia: a prospective multicenter study.

Infect Dis (Lond). 2024 Feb;56(2):128-137. doi: 10.1080/23744235.2023.2276785. Epub 2023 Dec 18.

Improving Suspected Pulmonary Infection Diagnosis by Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing: a Multicenter Retrospective Study.

Microbiol Spectr. 2022 Aug 31;10(4):e0247321. doi: 10.1128/spectrum.02473-21. Epub 2022 Aug 9.

A Case of Tuberculosis Misdiagnosed as Sarcoidosis and then Confirmed by NGS Testing.

Clin Lab. 2024 Mar 1;70(3). doi: 10.7754/Clin.Lab.2023.230823.

Tuberculosis-targeted next-generation sequencing and machine learning: An ultrasensitive diagnostic strategy for paucibacillary pulmonary tuberculosis and tuberculous meningitis.

Clin Chim Acta. 2024 Jan 15;553:117697. doi: 10.1016/j.cca.2023.117697. Epub 2023 Dec 23.

Diagnostic yield of Bronchoalveolar Lavage gene Xpert in smear-negative and sputum-scarce pulmonary tuberculosis.

J Coll Physicians Surg Pak. 2015 Feb;25(2):115-8.

引用本文的文献

Evaluation of targeted next-generation sequencing for microbiological diagnosis of acute lower respiratory infection.

Front Microbiol. 2025 Aug 18;16:1615965. doi: 10.3389/fmicb.2025.1615965. eCollection 2025.

A comprehensive evaluation of a novel targeted-sequencing workflow for species identification and anti-tuberculosis drug-resistance detection.

Front Cell Infect Microbiol. 2025 Jun 9;15:1584237. doi: 10.3389/fcimb.2025.1584237. eCollection 2025.

Targeted next-generation sequencing: a promising approach for detection and drug resistance when applied in paucibacillary clinical samples.

Microbiol Spectr. 2025 Jul;13(7):e0312724. doi: 10.1128/spectrum.03127-24. Epub 2025 Jun 10.

Identification of and its Drug Resistance by Targeted Nanopore Sequencing Technology.

Bio Protoc. 2025 Feb 5;15(3):e5170. doi: 10.21769/BioProtoc.5170.

Targeted next-generation sequencing for antimicrobial resistance detection in ventilator-associated pneumonia.

Front Cell Infect Microbiol. 2025 Jan 31;15:1526087. doi: 10.3389/fcimb.2025.1526087. eCollection 2025.

Field evaluation of nanopore targeted next-generation sequencing to predict drug-resistant tuberculosis from native sputum in South Africa and Zambia.

J Clin Microbiol. 2025 Mar 12;63(3):e0139024. doi: 10.1128/jcm.01390-24. Epub 2025 Feb 12.

Utilizing Targeted Next-Generation Sequencing for Rapid, Accurate, and Cost-Effective Pathogen Detection in Lower Respiratory Tract Infections.

Infect Drug Resist. 2025 Jan 17;18:329-340. doi: 10.2147/IDR.S494558. eCollection 2025.

Diagnostic Value of Targeted Next-generation Sequencing in Pulmonary Mycobacterial Infections.

Curr Med Sci. 2024 Oct;44(5):947-953. doi: 10.1007/s11596-024-2937-4. Epub 2024 Oct 5.

Exploring expression levels of the cGAS-STING pathway genes in peripheral blood mononuclear cells of spinal tuberculosis patients.

BMC Infect Dis. 2024 Sep 4;24(1):915. doi: 10.1186/s12879-024-09815-x.

Nanopore Sequencing Technology: A Reliable Method for Pathogen Diagnosis in Elderly Patients with Community-Acquired Pneumonia.

Infect Drug Resist. 2024 Aug 24;17:3659-3667. doi: 10.2147/IDR.S475861. eCollection 2024.

Application of targeted next generation sequencing technology in the diagnosis of Mycobacterium Tuberculosis and first line drugs resistance directly from cell-free DNA of bronchoalveolar lavage fluid.

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Application of targeted next generation sequencing technology in the diagnosis of Mycobacterium Tuberculosis and first line drugs resistance directly from cell-free DNA of bronchoalveolar lavage fluid.

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