Hatanaka H, Tsukui H, Nihonmatsu I
Department of Neuroscience, Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
Neurosci Lett. 1987 Aug 18;79(1-2):85-90. doi: 10.1016/0304-3940(87)90676-8.
The survival effect by nerve growth factor (NGF) on the cholinergic neurons of postnatal rat septal neurons in culture was examined. When the septal neurons from 10 to 12-day-old rats were cultured without NGF, the activities of choline acetyltransferase gradually decreased during the period of cultivation. The addition of NGF to the culture prevented the decline of activities. And, the number of acetylcholinesterase-positive neurons in culture with NGF was found to be more than that without NGF, after 5 days in culture. These results suggest that NGF promotes the survival of septal cholinergic neurons from postnatal rats in culture.
研究了神经生长因子（NGF）对培养的新生大鼠隔区神经元胆碱能神经元的存活作用。当培养来自10至12日龄大鼠的隔区神经元而不添加NGF时，胆碱乙酰转移酶的活性在培养期间逐渐降低。向培养物中添加NGF可防止活性下降。并且，培养5天后，发现添加NGF的培养物中乙酰胆碱酯酶阳性神经元的数量多于未添加NGF的培养物。这些结果表明，NGF可促进培养的新生大鼠隔区胆碱能神经元的存活。
