Experimental Morphology Laboratory, Morphology Department, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil; Medical Sciences Post Graduation Program, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil.
Experimental Morphology Laboratory, Morphology Department, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil; Medical Sciences Post Graduation Program, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil.
Morphologie. 2023 Jun;107(357):259-263. doi: 10.1016/j.morpho.2023.01.001. Epub 2023 Jan 25.
The present work aims to observe the clitoris' extracellular matrix in young and old women with a scanning electron microscope.
After approval of the local research ethics committee, samples of the clitoris body were obtained from cadavers of women between 20 and 40 old (G1) and from cadavers over the age of 60 (G2). The samples were decellularized with NaOH to maintain the extracellular matrix framework, submitted to silver sputter coating, and observed under a scanning electron microscope.
The mean age of the cadavers in G1 was 28 years old and 75±6 years old in G2. The groups were composed of 10 cadavers each. It was observed that the collagen was arranged in a disorganized fashion in the samples from the G2 in several regions. There was also a decrease in elastic fibers that anchored the collagen in these samples. The concentration of collagen showed an increase in the older samples in comparison to the G1 samples. Conclusions Female sexual dysfunction is a condition prevalent in a significantly large portion of women and it is more common in elderly women. It is known that the tumescence mechanism requires integrity of the extracellular matrix. The changes observed herein may alter the function of the organ and are similar to observations in studies of men with erectile dysfunction.
Female sexual dysfunction is a condition prevalent in a significantly large portion of women and it is more common in elderly women. It is known that the tumescence mechanism requires integrity of the extracellular matrix. The changes observed herein may alter the function of the organ and are similar to observations in studies of men with erectile dysfunction. .
本研究旨在使用扫描电子显微镜观察年轻和老年女性的阴蒂细胞外基质。
在获得当地研究伦理委员会的批准后，从 20 至 40 岁的女性（G1 组）和 60 岁以上的女性（G2 组）的尸体中获取阴蒂体样本。用 NaOH 对样本进行脱细胞处理以保持细胞外基质框架，然后进行银喷镀，并在扫描电子显微镜下进行观察。
G1 组的平均年龄为 28 岁，G2 组为 75±6 岁。每组包含 10 具尸体。结果显示，G2 组的多个区域中胶原排列紊乱。这些样本中的弹性纤维也减少了，从而固定了胶原。与 G1 组相比，较老的样本中的胶原浓度增加。结论：女性性功能障碍是一种在相当大比例的女性中普遍存在的疾病，并且在老年女性中更为常见。已知勃起机制需要细胞外基质的完整性。本文观察到的变化可能会改变器官的功能，与男性勃起功能障碍研究中的观察结果相似。
女性性功能障碍是一种在相当大比例的女性中普遍存在的疾病，并且在老年女性中更为常见。已知勃起机制需要细胞外基质的完整性。本文观察到的变化可能会改变器官的功能，与男性勃起功能障碍研究中的观察结果相似。
