Department of Chemistry, Faculty of Science, New Valley University, Alkharga, 72511, Egypt.
Department of Chemistry, Faculty of Science, Cairo University, Giza, 12613, Egypt.
Sci Rep. 2023 Jan 27;13(1):1502. doi: 10.1038/s41598-023-28402-9.
The primary objective of this study was to describe the cytotoxicity on HEPG-2 cells and to study the COVID‑19 activities of the novel HL ligand and its Cr and Cu nano-complexes. As well as exploring the chemistry of the prepared nano-complexes. In this paper novel Schiff base, N-(4, 6-dimethyl pyrimidin-2-yl)-4-(((2-hydroxyl naphthalene-1-y l) methylene) amino) benzene-sulfonamidesulfonyl) amide has been synthesized. The novel Schiff base HL is used to synthesize novel nano and micro-complexes with CrCl.6HO and CuCl.2HO. The prepared ligand and micro complexes were interpreted by different spectroscopic techniques. The nano-sized Cr and Cu complexes were synthesized in an environmentally friendly manner using Coriandrum sativum (CS) media extract in ethanol. The structure, morphologies and particle size of the nano-sized complexes were determined using FT-IR, TEM, and PXRD. The results showed that the nano-domain complexes are on the Sub-nano scale. Furthermore, using TGA, we studied the effect of heat on the size of newly prepared nano-complexes. Experimental data were supported by DFT calculations. The findings revealed that the metal complexes investigated are more stable than the free ligand HL. The antitumor activity was examined before and after heating the nano-complexes at 200 °C. The results reveal the Cr nano complex, after heating, exhibited strong antitumor activity with IC value (3.349 μg/ml). The tested Cu nano-complex shows good DNA cleavage. The liver cancer and COVID19 proteins were examined using molecular docking to identify the potential binding energy of inhibitors.
本研究的主要目的是描述对 HEPG-2 细胞的细胞毒性，并研究新型 HL 配体及其 Cr 和 Cu 纳米配合物的 COVID-19 活性。以及探索制备的纳米配合物的化学性质。在本文中，合成了新型希夫碱，N-（4,6-二甲基嘧啶-2-基）-4-（（2-羟基萘-1-基）亚甲基）氨基）苯磺酰胺磺酰基）酰胺。新型希夫碱 HL 用于与 CrCl.6HO 和 CuCl.2HO 合成新型纳米和微配合物。所制备的配体和微配合物通过不同的光谱技术进行解释。使用 Coriandrum sativum（CS）介质提取物在乙醇中以环保的方式合成纳米级 Cr 和 Cu 配合物。使用 FT-IR、TEM 和 PXRD 确定纳米级配合物的结构、形态和粒径。结果表明，纳米域配合物处于亚纳米级。此外，使用 TGA 研究了热对新制备的纳米配合物尺寸的影响。实验数据得到了 DFT 计算的支持。研究结果表明，所研究的金属配合物比游离配体 HL 更稳定。在将纳米配合物在 200°C 下加热前后测试了抗肿瘤活性。结果表明，加热后的 Cr 纳米配合物具有很强的抗肿瘤活性，IC 值（3.349μg/ml）。测试的 Cu 纳米配合物显示出良好的 DNA 切割活性。使用分子对接检查肝癌和 COVID19 蛋白，以确定抑制剂的潜在结合能。
