Biophysical Chemistry, Institute of Biochemistry, Felix-Hausdorff-Straße 4, University of Greifswald, 17489, Greifswald, Germany.
ZIK-HIKE, Zentrum für Innovationskompetenz "Humorale Immunreaktionen bei kardiovaskulären Erkrankungen,", Fleischmannstraße 42, 17489, Greifswald, Germany.
Macromol Biosci. 2023 Aug;23(8):e2200464. doi: 10.1002/mabi.202200464. Epub 2023 Feb 8.
Polystyrene is one of the most widely used plastics. This article reports on the interaction of 50 and 210 nm polystyrene nanoparticles (PSNPs) with human serum albumin (HSA) and transferrin (Tf), as well as their effect on supported lipid bilayers (SLBs), using experimental and theoretical approaches. Dynamic light scattering (DLS) and atomic force microscopy (AFM) measurements show that the increase in diameter for the PSNP-protein bioconjugates depends on nanoparticle size and type of proteins. The circular dichroism (CD) spectroscopy results demonstrate that the proteins preserve their structures when they interact with PSNPs at physiological temperatures. The quartz crystal microbalance (QCM) technique reveals that PSNPs and their bioconjugates show no strong interactions with SLBs. On the contrary, the molecular dynamics simulations (MDS) show that both proteins bind strongly to the lipid bilayer (SLBs) when compared to their binding to a polystyrene surface model. The interaction is strongly dependent on the protein and lipid bilayer composition. Both the PSNPs and their bioconjugates show no toxicity in human umbilical vein endothelial (HUVEC) cells; however, bare 210 nm PSNPs and 50 nm PSNP-Tf bioconjugates show an increase in reactive oxygen species production. This study may be relevant for assessing the impact of plastics on health.
聚苯乙烯是应用最广泛的塑料之一。本文采用实验和理论方法，研究了 50nm 和 210nm 聚苯乙烯纳米粒子（PSNPs）与人血清白蛋白（HSA）和转铁蛋白（Tf）的相互作用及其对支撑脂质双层（SLBs）的影响。动态光散射（DLS）和原子力显微镜（AFM）测量表明，PSNP-蛋白质结合物的直径增加取决于纳米颗粒的大小和蛋白质的类型。圆二色性（CD）光谱结果表明，蛋白质在生理温度下与 PSNPs 相互作用时保持其结构。石英晶体微天平（QCM）技术表明，PSNPs 及其生物缀合物与 SLBs 没有强烈的相互作用。相反，分子动力学模拟（MDS）表明，与聚苯乙烯表面模型相比，两种蛋白质与脂质双层（SLBs）的结合都很强。这种相互作用强烈依赖于蛋白质和脂质双层的组成。PSNPs 及其生物缀合物在人脐静脉内皮细胞（HUVEC）中均无毒性；然而，裸露的 210nm PSNPs 和 50nm PSNP-Tf 生物缀合物显示出活性氧物质产生的增加。这项研究可能对评估塑料对健康的影响具有重要意义。
