Graduate School of Biomedical Sciences, Tokushima University, Tokushima, Japan.
Department of Pathobiology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.
Lipids. 2023 Mar;58(2):93-103. doi: 10.1002/lipd.12366. Epub 2023 Jan 28.
Cancer cells are known to survive in a hypoxic microenvironment by altering their lipid metabolism as well as their energy metabolism. In this study, Caco-2 cells derived from human colon cancer, were found to have elevated intracellular levels of phosphatidic acid and its lysoform, lysophosphatidic acid (LPA), under hypoxic conditions. Our results suggested that the elevation of LPA in Caco-2 cells was mainly due to the combined increases in cellular levels of lysophosphatidylcholine and lysophosphatidylethanolamine by phospholipase A and subsequent hydrolysis to LPA by lysophospholipase D. We detected the Ca -stimulated choline-producing activities toward exogenous lysophosphatidylcholines in whole Caco-2 cell homogenates, indicating their involvement in the LPA production in intact Caco-2 cells.
癌细胞已知通过改变其脂代谢以及能量代谢在缺氧微环境中存活。在这项研究中，发现源自人结肠癌细胞的 Caco-2 细胞在缺氧条件下具有升高的磷脂酸及其溶血形式溶血磷脂酸 (LPA) 的细胞内水平。我们的结果表明，LPA 在 Caco-2 细胞中的升高主要是由于磷脂酶 A 导致细胞内溶血磷脂酰胆碱和溶血磷脂酰乙醇胺水平的联合增加，随后通过溶血磷脂酶 D 水解为 LPA。我们在整个 Caco-2 细胞匀浆中检测到对外源溶血磷脂酰胆碱的 Ca 刺激胆碱产生活性，表明它们参与完整的 Caco-2 细胞中 LPA 的产生。
