Ministry of Health, Northern State, Dongola, Sudan.
Department of Medical Microbiology, Faculty of Medical Laboratory Science, University of Gezira, Wad Medani, Sudan.
Asian Pac J Cancer Prev. 2023 Jan 1;24(1):21-24. doi: 10.31557/APJCP.2023.24.1.21.
Leukemia frequently causes anemia; thus patients need products containing normal blood or RBCs for treatment. Anti-red blood cell alloantibody formation is still a critical challenge in transfusion.
The current study aimed to investigate RBC alloantibodies in Sudanese leukemia patients who receive multiple blood transfusions at the cancer center in Dongola and Maroyee, Northern Sudan.
At the Northern State oncology center in Dongola and Maroyee, Sudan, an across-sectional descriptive study design was used. In this study, 100 leukemic patients who had received blood transfusions three times or more were enrolled. From each participant, Peripheral blood was drawn in amounts of 3 ml in EDTA vacutainer tubes for ABO blood group and Rh factor testing and 3 ml in non-additive containers for antibody screening and Alloantibody identification. All individuals' ABO blood groups and Rh factors were determined using the slide method. Indirect Coombs test apply to detect alloantibodies by Polly Specific antihuman globulin reagents using tube method techniques. Alloantibody identification was performed by DiaMed-ID microtyping system.
Incidence of alloimmunization was 11%, with 11 alloantibodies found in 11 patients. The most common alloantibody was kell (36.4%), followed by Lea (27.2%; 3/11), then P (18.2%; 2/11) and M (18.2%; 2/11).
Anti kell antibody was the most prevalent alloantibody among leukemic patients with multiple transfusions.
白血病常导致贫血；因此，患者需要接受含有正常血液或 RBC 的产品进行治疗。抗红细胞同种异体抗体的形成仍然是输血中的一个关键挑战。
本研究旨在调查苏丹北部东哥拉和马鲁耶癌症中心接受多次输血的白血病患者的 RBC 同种异体抗体。
在苏丹北部州肿瘤中心，采用横断面描述性研究设计。本研究纳入了 100 名接受过 3 次或以上输血的白血病患者。从每个参与者中抽取 3 毫升外周血于 EDTA 真空采血管中进行 ABO 血型和 Rh 因子检测，抽取 3 毫升于非添加剂容器中进行抗体筛查和同种异体抗体鉴定。所有个体的 ABO 血型和 Rh 因子均采用玻片法确定。间接 Coombs 试验采用 Polly 特异性抗人球蛋白试剂通过试管法技术检测同种异体抗体。同种异体抗体鉴定采用 DiaMed-ID 微分型系统进行。
同种免疫的发生率为 11%，在 11 名患者中发现了 11 种同种异体抗体。最常见的同种异体抗体是 kell（36.4%），其次是 Lea（27.2%；3/11），然后是 P（18.2%；2/11）和 M（18.2%；2/11）。
在多次输血的白血病患者中，抗 kell 抗体是最常见的同种异体抗体。
