Department of digestive system, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, No. 445, Bayi Avenue, Nanchang, 330000, Jiangxi Province, China.
Center of digestive endoscopy, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, No. 445, Bayi Avenue, Nanchang, 330000, Jiangxi Province, China.
BMC Complement Med Ther. 2023 Jan 28;23(1):23. doi: 10.1186/s12906-023-03851-x.
Acute pancreatitis (AP) is one of the most common acute abdomen. Inflammation and apoptosis are closely linked with AP development. Total flavonoids of Chrysanthemum indicum L (TFC) has been proved to inhibit inflammation and apoptosis. If TFC could suppress AP remains unclear. AP animal and cell models were established with Cerulein. The pancreatic tissue injury was measured with HE staining. Inflammatory factors were detected with ELISA method. The protein expression was evaluated with Western blotting. Inhibition of AP in vivo was achieved by TFC by inhibiting serum amylase, myeloperoxidase (MPO), and water content of pancreatic tissue. The increased inflammatory response and activation of NF-κB signaling pathway in AP rats were inhibited after TFC treatment. The activation of NF-κB signaling pathway, increase of cell apoptosis and inflammatory factors in AR42J cells were suppressed by TFC. We demonstrated that TFC could significantly inhibit AP through restraining serum amylase, MPO, water content of pancreatic tissue, inflammation levels, apoptosis, and NF-κB signaling pathway activation. This study might clarify the potential inhibition mechanism of TFC in AP development.
急性胰腺炎（AP）是最常见的急腹症之一。炎症和细胞凋亡与 AP 的发展密切相关。野菊花总黄酮（TFC）已被证明能抑制炎症和细胞凋亡。然而，TFC 是否能抑制 AP 尚不清楚。用胆酸钠建立 AP 动物和细胞模型。用 HE 染色法测量胰腺组织损伤。用 ELISA 法检测炎症因子。用 Western blot 法评价蛋白表达。TFC 通过抑制血清淀粉酶、髓过氧化物酶（MPO）和胰腺组织含水量来抑制体内 AP。TFC 处理后，AP 大鼠的炎症反应增强和 NF-κB 信号通路激活受到抑制。TFC 抑制 AR42J 细胞中 NF-κB 信号通路的激活、细胞凋亡和炎症因子的增加。我们的研究表明，TFC 通过抑制血清淀粉酶、MPO、胰腺组织含水量、炎症水平、细胞凋亡和 NF-κB 信号通路激活，显著抑制 AP。本研究可能阐明了 TFC 在 AP 发展中的潜在抑制机制。
