Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Guangzhou, 510642, Guangdong, China.
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong, China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Guangzhou, 510642, Guangdong, China.
Poult Sci. 2023 Apr;102(4):102469. doi: 10.1016/j.psj.2022.102469. Epub 2023 Jan 9.
The yellow color of the skin is an important economic trait for yellow chickens. Low and non-uniform skin yellowness would reduce economic efficiency. However, the regulatory mechanism of chicken skin yellowness has not been fully elucidated. In this study, we evaluated the skin yellowness of 819 chickens by colorimeter and digital camera, which are from the same batch and the same age of 2 pure lines with significant differences in skin yellowness. A total of 982 candidate differential expressed genes (DEGs) were detected in duodenal tissue by RNA-seq analysis for high and low yellowness chickens. Among the DEGs, we chose fatty acid translocase (CD36) gene and identified a single nucleotide polymorphism (SNP) upstream of the CD36 gene that was significantly associated with skin yellowness at multiple parts of the chicken, and its different genotypes had significant effects on the promoter activity of the CD36 gene. These findings will help to further elucidate the molecular mechanism of chicken skin yellowness and is helpful for improving chicken skin yellowness.
皮肤的黄色是黄鸡的一个重要经济性状。皮肤黄色度低且不均匀会降低经济效益。然而，鸡皮肤黄色的调控机制尚未完全阐明。在这项研究中，我们通过色度计和数码相机评估了 819 只来自同一批、同一年龄的 2 个纯系鸡的皮肤黄色度，这 2 个纯系鸡的皮肤黄色度有显著差异。通过对高、低黄色度鸡的十二指肠组织进行 RNA-seq 分析，共检测到 982 个候选差异表达基因（DEGs）。在 DEGs 中，我们选择了脂肪酸转运蛋白（CD36）基因，并鉴定出 CD36 基因上游的一个单核苷酸多态性（SNP），该 SNP 与鸡体多个部位的皮肤黄色度显著相关，其不同基因型对 CD36 基因的启动子活性有显著影响。这些发现将有助于进一步阐明鸡皮肤黄色度的分子机制，并有助于提高鸡皮肤黄色度。
