Department of Analytical, Bioanalytical Sciences, and Miniaturization, UMR 8231 Chemistry, Biology and Innovation, ESPCI Paris, PSL Research University, CNRS, 10 rue Vauquelin, 75005 Paris, France; Laboratoire Détection, Capteurs et Mesures, Ifremer, Centre Bretagne, Technopole pointe du diable, 29280 Plouzané, France.
Department of Analytical, Bioanalytical Sciences, and Miniaturization, UMR 8231 Chemistry, Biology and Innovation, ESPCI Paris, PSL Research University, CNRS, 10 rue Vauquelin, 75005 Paris, France; Sorbonne University, 75005 Paris, France.
Talanta. 2023 May 1;256:124295. doi: 10.1016/j.talanta.2023.124295. Epub 2023 Jan 20.
Several ion-imprinted polymers (IIPs) were synthesized via bulk polymerization with Cu(II) as template ion, methacrylic acid as functional monomer, ethylene glycol dimethacrylate as crosslinking agent, and azobisisobutyronitrile as initiator in acetonitrile or methanol as porogen solvent. Non-imprinted polymers (NIPs) were similarly synthesized but without Cu(II). After grounding and sieving, the template ions were removed from IIPs particles through several cycles of elimination in 3 M HCl. All NIPs were equally subjected to this acid treatment with the exception of one NIP, called unwashed NIP. The resulting IIP/NIP particles were packed in solid phase extraction (SPE) cartridges for characterization. The SPE protocol was designed by optimizing a washing step following the sample percolation to eliminate potential interfering ions prior to the elution of Cu(II), all fractions analyzed by inductively coupled plasma mass spectrometry. The best IIP showed a high specificity (recovery of Cu(II) vs. interfering ions) and a good selectivity (retention on IIP vs. NIP). Its adsorption capacity was determined to be 63 μg g. Then, a volume of 50 mL was percolated with 30 mg of IIP, thus giving rise to an enrichment factor of 24. Finally, applications to real samples (mineral and sea waters) were successfully performed. In addition, Brunauer-Emmett-Teller analyses showed that the surface area of the washed NIP was almost double that of the unwashed one (140.70 vs. 74.49 m g), demonstrating for the first time that the post-treatment of a NIP after its synthesis may have a significant impact on its porous structure, and thus need to be more precisely detailed by authors in the future papers.
几种离子印迹聚合物（IIPs）通过本体聚合合成，Cu（II）作为模板离子，甲基丙烯酸作为功能单体，乙二醇二甲基丙烯酸酯作为交联剂，偶氮二异丁腈作为引发剂，在乙腈或甲醇中作为致孔剂溶剂。非印迹聚合物（NIPs）也是通过类似的方法合成的，但不使用 Cu（II）。研磨和筛分后，通过在 3M HCl 中进行几次洗脱循环，从 IIP 颗粒中去除模板离子。所有的 NIPs 都要经过这种酸处理，除了一个称为未清洗 NIP 的 NIP 之外。得到的 IIP/NIP 颗粒被填充在固相萃取（SPE）小柱中进行表征。SPE 方案是通过优化样品渗滤后的洗涤步骤设计的，以在洗脱 Cu（II）之前消除潜在的干扰离子，所有的洗脱液都通过电感耦合等离子体质谱进行分析。最佳的 IIP 表现出高特异性（Cu（II）的回收率与干扰离子的回收率）和良好的选择性（在 IIP 上的保留与在 NIP 上的保留）。其吸附容量为 63μg/g。然后，用 30mg IIP 渗滤 50mL 体积，从而得到 24 的富集因子。最后，成功地应用于实际样品（矿化水和海水）。此外，Brunauer-Emmett-Teller 分析表明，经过洗涤的 NIP 的表面积几乎是未洗涤的 NIP 的两倍（140.70 对 74.49 m²/g），这首次表明，在合成 NIP 后对其进行后处理可能会对其多孔结构产生重大影响，因此，作者在未来的论文中需要更精确地详细说明。
