Kim Sunhyung, Krishnan Hari B
Division of Plant Science and Technology, University of Missouri, Columbia, MO, United States.
Division of Plant Science and Technology, University of Missouri, Columbia, MO, United States; Plant Genetics Research Unit, USDA, Agricultural Research Service, Columbia, MO, United States.
Methods Enzymol. 2023;680:195-213. doi: 10.1016/bs.mie.2022.08.016. Epub 2022 Sep 13.
Rapid and accurate measurement of trypsin inhibitor is critical for soy processors to assess the quality of soy meal. Currently, trypsin inhibitor activity is measured using the American Oil Chemists' Society (AOCS) and the American Association of Cereal Chemists International (AACCI) approved method. We have modified and improved the AACCI/AOCS approved method resulting in the elimination of several time-consuming steps and drastically reducing the assay volume. By employing our simplified procedure, we have measured trypsin inhibitor activity of several soy and soy products. A side-by side comparison of our simplified procedure with AOCS approved method revealed strikingly similar results indicating that several time-consuming and tedious steps associated with AACCI/AOCS approved methods can be eliminated without sacrificing the accuracy of the assay. Moreover, we demonstrate that our assay can also be carried out in 96-well microplates which will enable high-throughput screening of large number of soy meal samples.
快速准确地测定胰蛋白酶抑制剂对大豆加工商评估豆粕质量至关重要。目前，胰蛋白酶抑制剂活性是使用美国油脂化学家协会（AOCS）和美国谷物化学家协会国际分会（AACCI）批准的方法来测定的。我们对AACCI/AOCS批准的方法进行了修改和改进，省去了几个耗时的步骤，并大幅减少了测定体积。通过采用我们简化的程序，我们测定了几种大豆及大豆制品的胰蛋白酶抑制剂活性。将我们的简化程序与AOCS批准的方法进行并行比较，结果显示出惊人的相似性，这表明与AACCI/AOCS批准方法相关的几个耗时且繁琐的步骤可以省去，而不会牺牲测定的准确性。此外，我们证明我们的测定也可以在96孔微孔板中进行，这将能够对大量豆粕样品进行高通量筛选。
