PHIM, CEFE, Institut Agro, INRAE, CIRAD, Univ Montpellier, 34000, Montpellier, France.
PHIM, INRAE, CIRAD, Institut Agro, Univ Montpellier, 34000, Montpellier, France.
New Phytol. 2023 Apr;238(2):835-844. doi: 10.1111/nph.18778. Epub 2023 Feb 18.
Reports indicate that intraspecific neighbours alter the physiology of focal plants, and with a few exceptions, their molecular responses to neighbours are unknown. Recently, changes in susceptibility to pathogen resulting from such interactions were demonstrated, a phenomenon called neighbour-modulated susceptibility (NMS). However, the genetics of NMS and the associated molecular responses are largely unexplored. Here, we analysed in rice the modification of biomass and susceptibility to the blast fungus pathogen in the Kitaake focal genotype in the presence of 280 different neighbours. Using genome-wide association studies, we identified the loci in the neighbour that determine the response in Kitaake. Using a targeted transcriptomic approach, we characterized the molecular responses in focal plants co-cultivated with various neighbours inducing a reduction in susceptibility. Our study demonstrates that NMS is controlled by one major locus in the rice genome of its neighbour. Furthermore, we show that this locus can be associated with characteristic patterns of gene expression in focal plant. Finally, we propose an hypothesis where Pi could play a role in explaining this case of NMS. Our study sheds light on how plants affect the physiology in their neighbourhood and opens perspectives for understanding plant-plant interactions.
报告表明，种内邻居会改变焦点植物的生理学特性，除了少数例外，它们对邻居的分子反应尚不清楚。最近，由于这种相互作用，植物对病原体的易感性发生了变化，这种现象被称为邻域调节易感性（NMS）。然而，NMS 的遗传学及其相关的分子反应在很大程度上仍未被探索。在这里，我们在水稻中分析了在存在 280 种不同邻居的情况下，焦点基因型 Kitaake 生物量和对稻瘟病菌病原体易感性的改变。使用全基因组关联研究，我们确定了决定 Kitaake 反应的邻居中的基因座。使用靶向转录组学方法，我们描述了与各种诱导易感性降低的邻居共培养的焦点植物中的分子反应。我们的研究表明，NMS 由其邻居的水稻基因组中的一个主要基因座控制。此外，我们表明，该基因座可以与焦点植物中特征性的基因表达模式相关联。最后，我们提出了一个假设，即 Pi 可能在解释这种 NMS 情况中发挥作用。我们的研究揭示了植物如何影响其周围环境的生理学特性，并为理解植物-植物相互作用开辟了前景。
