Investigation of Curcumin-Loaded OA400 Nanoparticle's Effect on the Expression of and Human Papilloma-Virus Oncogenes and and Factors in HeLa Cell Line.

BACKGROUND

Curcumin, a compound derived from the root of the , has been confirmed as an anticancer, chemoprotective, and gene/protein regulatory agent. Nanoformulation of curcumin has been developed to increase its targeting efficiency, solubility, controlled release, and physical and chemical stability.

OBJECTIVES

This study investigated the effect of new nano-type curcumin, oleic acid-derived dendrosome (OA400 nanoparticles), on the expression of and human papillomavirus oncogenes and and factors in the HeLa cell line. After preparing nano-curcumin by mixing OA400 nano-carrier and curcumin, its effect was considered on the human cervical cancer cell line (HeLa cell line RRID: CVCL_003) and normal fibroblast cells.

METHODS

MTT assay and flow cytometry were used to evaluate cell viability and apoptosis. Furthermore, real-time RT-PCR and western blot analyses assessed RNA and protein expression of , , , and . Statistical analyses were performed by GraphPad Prism 7 software.

RESULTS

The nanoformulation of curcumin could reduce the expression of and oncogenes and increase and tumor suppressors in HeLa cancerous cells at 15 μM concentration; however, it had no significant effect on the viability of normal fibroblast cells. On the other hand, curcumin altered the expression of these genes at a 50-μM concentration. Gene and protein expression analysis indicated the up-regulation of and factors and the down-regulation of and under the influence of nano-curcumin treatment more than curcumin.

CONCLUSIONS

These data indicate the potential of curcumin-loaded OA400 nanoparticles to be considered as a treatment option in cervical cancer investigations.