Li Zhirong, Wu Yipeng, Zhang Cong, Dai Suli, Wei Sisi, Zhao Ruinian, Gao Feng, Zhao Lianmei, Shan Baoen
Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China.
Provincial Center for Clinical Laboratories, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, China.
J Oncol. 2023 Jan 19;2023:3335959. doi: 10.1155/2023/3335959. eCollection 2023.
Long noncoding RNAs (lncRNAs) are gradually being annotated as important regulators of multiple cellular processes. The goal of our study was to investigate the effects of the lncRNA small nucleolar RNA host gene 5 (SNHG5) in lung adenocarcinoma (LAD) and its underlying mechanisms. The findings revealed a substantial drop in SNHG5 expression in LAD tissues, which correlated with clinical-pathological parameters. Transcriptome sequencing analysis demonstrated that the inhibitory effect of SNHG5 was associated with cell adhesion molecules. Moreover, the expression of SNHG5 was shown to be correlated with epithelial-mesenchymal transition (EMT) markers in western blots and immunofluorescence. SNHG5 also had significant effects of antimigration and anti-invasion on LAD cells . Furthermore, the migration and invasion of A549 cells were suppressed by overexpressed SNHG5 in the EMT progress induced by transforming growth factor 1 (TGF-1), and this might be due to the inhibition of the expression of EMT-associated transcription factors involving Snail, SLUG, and ZEB1. In LAD tissues, the expression of SNHG5 exhibited a positive association with E-cadherin protein expression but a negative correlation with N-cadherin and vimentin, according to the results of quantitative real-time PCR (qRT-PCR). In summary, the current work demonstrated that the lncRNA SNHG5 might limit cell migration and invasion of LAD cancer via decreasing the EMT process, indicating that SNHG5 might be used as a target for LAD therapeutic methods.
长链非编码RNA（lncRNAs）正逐渐被认定为多种细胞过程的重要调节因子。我们研究的目的是探究长链非编码RNA小核仁RNA宿主基因5（SNHG5）在肺腺癌（LAD）中的作用及其潜在机制。研究结果显示，LAD组织中SNHG5的表达显著下降，这与临床病理参数相关。转录组测序分析表明，SNHG5的抑制作用与细胞粘附分子有关。此外，蛋白质免疫印迹和免疫荧光结果显示，SNHG5的表达与上皮-间质转化（EMT）标志物相关。SNHG5对LAD细胞的迁移和侵袭也有显著抑制作用。此外，在转化生长因子1（TGF-1）诱导的EMT过程中，过表达的SNHG5抑制了A549细胞的迁移和侵袭，这可能是由于抑制了与EMT相关的转录因子Snail、SLUG和ZEB1的表达。根据定量实时聚合酶链反应（qRT-PCR）结果，在LAD组织中，SNHG5的表达与E-钙粘蛋白的蛋白表达呈正相关，但与N-钙粘蛋白和波形蛋白呈负相关。总之，目前的研究表明，lncRNA SNHG5可能通过减少EMT过程来限制LAD癌细胞的迁移和侵袭，这表明SNHG5可能成为LAD治疗方法的一个靶点。
