Flamier Anthony, Bisht Punam, Richards Alexsia, Tomasello Danielle, Jaenisch Rudolf
bioRxiv. 2023 Jan 10:2023.01.10.523422. doi: 10.1101/2023.01.10.523422.
COVID-19 has impacted billions of people in the world since 2019 and unfolded a major healthcare crisis. With an increasing number of deaths and the emergence of more transmissible variants, it is crucial to better understand the biology of the disease-causing virus, the SARS-CoV-2. Peripheral neuropathies appeared as a specific COVID-19 symptom occurring at later stages of the disease. In order to understand the impact of SARS-CoV-2 on the peripheral nervous system, we generated human sensory neurons from induced pluripotent stem cells that we infected with the SARS-CoV-2 strain WA1/2020 and the variants delta and omicron. Using single cell RNA sequencing, we found that human sensory neurons can be infected by SARS-CoV-2 but are unable to produce new viruses. Our data suggests that sensory neurons can be infected by the original WA1/2020 strain of SARS-CoV-2 as well as the delta and omicron variants.
自2019年以来，新冠病毒已影响全球数十亿人，并引发了一场重大的医疗危机。随着死亡人数的增加以及更具传播性的变种的出现，更好地了解致病病毒——严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的生物学特性至关重要。周围神经病变作为新冠病毒感染后期出现的一种特定症状而显现。为了了解SARS-CoV-2对周围神经系统的影响，我们从诱导多能干细胞中生成了人类感觉神经元，并将其感染SARS-CoV-2毒株WA1/2020以及德尔塔和奥密克戎变种。通过单细胞RNA测序，我们发现人类感觉神经元可被SARS-CoV-2感染，但无法产生新的病毒。我们的数据表明，感觉神经元可被SARS-CoV-2的原始WA1/2020毒株以及德尔塔和奥密克戎变种感染。
